The effect of anesthetics on mesangial cells proliferation via protein kinase pathway.

麻醉剂通过蛋白激酶途径对系膜细胞增殖的影响。

• 批准号: 09671595
• 负责人: SHIGEMATSU Akio
• 金额: $ 2.18万
• 依托单位: University of Occupational Environmental Health(UOEH)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671595/
• 关键词: ketamine; propofol; mesangial cell; [^3H]tymidine; Protein Kinase; 5HT2A; Muscarinic reseptor; Xenopus oocytes; protein kinase C; 静脈麻酔薬; ラット腎メサンギウム細胞; アフリカツメガエル卵母細胞

We studied the effects of intravenous anesthetics ketamine and propofol on rat mesangial cell (MC) proliferation.(1)Ketamine inhibited [^3Hltymidine incorporation into the mesangial cells in a concentration-dependent manner (10 mM 1 mM). But, Propofol had no effects n [3H]tyrnidine incorporation.(2)Ketamine also attenuated the cell number at clinical concentrations and increased cAMP levels in MC.(3)Protein Kinase A inhibitor recoverd the inhibition of ketamine on MC proliferation.(4)Moreover, anesthetics (halothane and isoflurane) inhibited the 5HT2A and Muscarinic receptor type1 function via Protein kinase C pathway in Xenopus oocytes. These results suggested that anesthetics have effects on protein kinase activity in the cells and regulated the MC proliferation and receptor function via protein kinase.

本文研究了静脉麻醉药氯胺酮和异丙酚对大鼠系膜细胞增殖的影响。（1）氯胺酮以浓度依赖性方式（10 mM 1 mM）抑制[^3H]-胸苷掺入系膜细胞。异丙酚对[3 H]嘧啶核苷掺入无影响。（2）临床浓度的氯胺酮可使MC细胞数减少，cAMP水平升高。（3）蛋白激酶A抑制剂可恢复氯胺酮对MC增殖的抑制作用。（4）氟烷和异氟烷通过蛋白激酶C途径抑制爪蟾卵母细胞5 HT 2A和M受体1的功能。这些结果表明，麻醉药对细胞内蛋白激酶活性有影响，并通过蛋白激酶调节MC增殖和受体功能。

项目成果

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