The effect of anesthetics on mesangial cells proliferation via protein kinase pathway.

本文研究了静脉麻醉药氯胺酮和异丙酚对大鼠肾小球系膜细胞(MC)增殖的影响：(1)氯胺酮以浓度依赖方式(10 mM~1 mM)抑制[~3Hltymidine]掺入系膜细胞；(2)氯胺酮还可减少临床浓度下MC的细胞数，升高cAMP水平。(3)蛋白激酶A抑制剂可恢复氯胺酮对MC增殖的抑制作用。(4)麻醉剂(氟烷和异氟烷)通过蛋白激酶C途径抑制非洲爪哇卵母细胞5HT2A和M受体1的功能。提示麻醉药对细胞内蛋白激酶活性有影响，并通过蛋白激酶调节MC的增殖和受体功能。

We studied the effects of intravenous anesthetics ketamine and propofol on rat mesangial cell (MC) proliferation.(1)Ketamine inhibited [^3Hltymidine incorporation into the mesangial cells in a concentration-dependent manner (10 mM 1 mM). But, Propofol had no effects n [3H]tyrnidine incorporation.(2)Ketamine also attenuated the cell number at clinical concentrations and increased cAMP levels in MC.(3)Protein Kinase A inhibitor recoverd the inhibition of ketamine on MC proliferation.(4)Moreover, anesthetics (halothane and isoflurane) inhibited the 5HT2A and Muscarinic receptor type1 function via Protein kinase C pathway in Xenopus oocytes. These results suggested that anesthetics have effects on protein kinase activity in the cells and regulated the MC proliferation and receptor function via protein kinase.