Immunohistochemical study of click sensitivity of the vestibular system

前庭系统咔嗒声敏感性的免疫组化研究[导读]神经生理学研究表明，哺乳动物前庭神经元对响亮的咔嗒声有反应。这些研究支持了人类咔哒声诱发的肌源性电位可能起源于前庭。虽然单个单元的研究为前庭神经元对点击的敏感性提供了有力的证据，但要了解前庭系统中点击敏感神经元的分布并不容易。免疫组化研究将提供补充结果，在此，我们报告了o-Fos蛋白诱导前庭系统敏感性的免疫组化结果。【材料与方法】成年大鼠（180 ~ 200 g）接受高于ABR阈值90 dB的点击刺激，持续40分钟。刺激后，用卡莫夫斯基溶液（2.5%多聚甲醛和0.4%戊二醛）灌注固定大鼠。将冷冻的大鼠脑干切成冠状切片。对标本进行c-Fos蛋白反应。第一抗体为抗c- fos lgG（兔）。用生物素化抗兔lgG（痛风）和亲和素-生物素化过氧化物酶检测组织偶联抗血清，然后用二氨基联苯胺和过氧化氢可视化过氧化物酶。[结果]耳蜗核、上橄榄、梯形体、下丘等听觉通路c-Fos蛋白表达强烈。在前庭核中，c-Fos蛋白在前庭核外侧、脊髓和内侧被诱导。[结论]这些结果证实了前庭神经元对咔哒声的敏感性。

Immunohistochemical study of click-sensitivity of the vestibular system[Introduction] Neurophysiological studies revealed that vestibular neurons in mammals could respond to loud click. These studies have supported that click-evoked myogenic potentials in humans could be of vestibular origin. While single unit studies provide a strong evidence of sensitiveness of vestibular neurons to click, it is not easy to get knowledge about distribution of click-sensitive neurons in the vestibular system. Immunohistochemical study would provide complemental findings, Herein we report immunohistochemical findings about sensitiveness of the vestibular system by o-Fos protein induction. [Materials ＆ Methods] Adult wister rats (180 to 200 g) received click stimulation of 90 dB above the ABR threshold for 40 minutes. After the stimulation, rats were perfused and fixed by Kamovsky's solution (2.5% paraformaldehyde and 0.4% glutaraldehyde). Frozen brainstems of rats were cut into coronal sections. The specimens were reacted for c-Fos protein. First antibody was anti-c-Fos lgG (rabbit). The tissue-conjugated anti-serum was detected with biotinylated anti-rabbit lgG (gout) and avidin-biotinylated peroxidase followed by the visualization of peroxydase with diaminobenzidine and hydrogen peroxide. [Results] The auditory pathway including the cochlear nuclei, the superior olive, the trapezoid body, the inferior colliculi showed strong induction at c-Fos protein. In the vestibular nuclei, induction of c-Fos protein was observed in the lateral, spinal, and medial vestibular nuclei. [Conclusion] These results confirmed neurophysiological studies concerning sensitiveness or vestibular neurons to click.