Label-free volumetric microscopy for visualizing intracellular motility of tumor cell culture

用于可视化肿瘤细胞培养物细胞内运动的无标记体积显微镜

• 批准号: 22KF0058
• 负责人: 安野 嘉晃
• 金额: $ 1.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0058/
• 关键词: Dynamic OCT; Tumor spheroid; Anti-cancer drug test; Label-free oncology; Cancer diagnosis

In FY 2022, the proposed OCT microscope has been successfully used for the anti-cancer drug investigations of the tumor spheroid. Three anti-cancer drugs were applied to human breast cancer (MCF-7) spheroids and the dynamic OCT microscopic images revealed totally different response patterns among the applied drugs. In addition, the dynamic OCT patterns were consistent with the standard fluorescence microscopic images. The results may proof the feasibility of our proposed to perform anti-cancer drug investigations.The above mentioned results have been published as an oral and poster presentations in an international conference (Photonics West 2023, BiOS conference) and a journal paper is now under writing. Several co-authored work were published in the same conference. In addition, a co-authored paper about dynamic optical coherence tomography based evaluation of human derived alveolar organoids has been published in Biomedical Optics Express journal.Further development of our proposed microscope has been also performed during FY 2022. It includes allowing the longitudinal imaging ability of our microscope. This has been performed by integrating a cultivation chamber with the microscope to allow the simulations tissue activity imaging during cultivation. The cultivation chamber integration was successful so far. And we successfully obtained the D-OCT images though the cultivation chamber window and the cap of the well plate containing the samples. It will allow future longitudinal drug response evaluation of tumor spheroids and fresh ex-vivo tissue.

于2022财政年度，建议的OCT显微镜已成功用于肿瘤球体的抗癌药物研究。将三种抗癌药物应用于人乳腺癌（MCF-7）球体，动态OCT显微图像显示所应用的药物之间完全不同的反应模式。此外，动态OCT图案与标准荧光显微图像一致。上述结果已在国际会议（Photonics West 2023，BiOS conference）上以口头和海报形式发表，目前正在撰写期刊论文。在同一次会议上发表了几项共同撰写的工作。此外，一篇关于基于动态光学相干断层扫描评估人类衍生肺泡类器官的合著论文已发表在Biomedical Optics Express期刊上。它包括允许我们的显微镜的纵向成像能力。这已经通过将培养室与显微镜集成来进行，以允许在培养期间模拟组织活动成像。 修炼室的整合，到目前为止，还是成功的。通过培养室窗口和装有样品的孔板盖，我们成功地获得了D-OCT图像。它将允许未来对肿瘤球体和新鲜离体组织进行纵向药物反应评价。

项目成果

National Taiwan University/Chang Gung University(その他の国・地域)

国立台湾大学/长庚大学（其他国家/地区）

Non-invasive and label-free zebrafish investigations using polarization-sensitive optical coherence tomography

使用偏振敏感光学相干断层扫描进行非侵入性和无标记斑马鱼研究

• 发表时间: 2023
• 作者: Antonia Lichtenegger,Lida Zhu;Tomoko Mori;Rion Morishita;Lixuan Bian;Ibrahim Abd El-Sadek;Shuichi Makita;Satoshi Matsusaka,Makoto Kobayashi,Yoshiaki Yasuno
• 通讯作者: Satoshi Matsusaka,Makoto Kobayashi,Yoshiaki Yasuno

Low- and high-resolution volumetric dynamic optical coherence tomography of in vitro cell cultures

体外细胞培养物的低分辨率和高分辨率体积动态光学相干断层扫描

• 发表时间: 2023
• 作者: Rion Morishita;Ibrahim Abd El-Sadek;Pradipta Mukherjee;Tomoko Mori;Suzuki Toshio;Antonia Lichtengger;Lim Yi Heng;Zhu Yiqiang;Shuichi Makita;Yuki Yamamoto;Tetsuharu Nagamoto;Satoshi Matsusaka;Yoshiaki Yasuno
• 通讯作者: Yoshiaki Yasuno

Medical University of Vienna(オーストリア)

维也纳医科大学（奥地利）

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