Label-free volumetric microscopy for visualizing intracellular motility of tumor cell culture

在2022财年，提出的OCT显微镜已成功用于肿瘤球体的抗癌药物研究。将三种抗癌药物应用于人乳腺癌(MCF-7)球体，动态OCT显微图像显示出不同药物之间的完全不同的反应模式。此外，动态OCT图像与标准荧光显微图像一致。上述结果已经以口头和海报的形式在一次国际会议(Photonics West 2023，Bios Conference)上发表，目前正在撰写一篇期刊论文。在同一次会议上发表了几部合著的作品。此外，在《生物医学光学快报》杂志上发表了一篇关于基于动态光学相干层析技术的人类衍生肺泡有机体评估的合著论文。我们所提出的显微镜的进一步开发也在2022财年进行。它包括允许我们的显微镜的纵向成像能力。这是通过将培养室与显微镜相结合来实现的，以允许在培养过程中模拟组织活动成像。到目前为止，培养箱整合成功。并通过培养腔窗口和装有样品的井板盖成功地获得了D-OCT图像。它将允许未来对肿瘤球体和新鲜的体外组织进行纵向药物反应评估。

In FY 2022, the proposed OCT microscope has been successfully used for the anti-cancer drug investigations of the tumor spheroid. Three anti-cancer drugs were applied to human breast cancer (MCF-7) spheroids and the dynamic OCT microscopic images revealed totally different response patterns among the applied drugs. In addition, the dynamic OCT patterns were consistent with the standard fluorescence microscopic images. The results may proof the feasibility of our proposed to perform anti-cancer drug investigations.The above mentioned results have been published as an oral and poster presentations in an international conference (Photonics West 2023, BiOS conference) and a journal paper is now under writing. Several co-authored work were published in the same conference. In addition, a co-authored paper about dynamic optical coherence tomography based evaluation of human derived alveolar organoids has been published in Biomedical Optics Express journal.Further development of our proposed microscope has been also performed during FY 2022. It includes allowing the longitudinal imaging ability of our microscope. This has been performed by integrating a cultivation chamber with the microscope to allow the simulations tissue activity imaging during cultivation. The cultivation chamber integration was successful so far. And we successfully obtained the D-OCT images though the cultivation chamber window and the cap of the well plate containing the samples. It will allow future longitudinal drug response evaluation of tumor spheroids and fresh ex-vivo tissue.