Label-free volumetric microscopy for visualizing intracellular motility of tumor cell culture

在2022财年，所提出的OCT显微镜已成功用于肿瘤球体的抗癌药物研究。将三种抗癌药物应用于人乳腺癌（MCF-7）球体，动态OCT显微镜图像显示不同药物的反应模式完全不同。此外，动态OCT模式与标准荧光显微镜图像一致。结果可能证明我们提出的进行抗癌药物研究的可行性。上述结果已在国际会议（Photonics West 2023, BiOS conference）上以口头和海报形式发表，目前正在撰写期刊论文。在同一会议上发表了几篇合著的论文。此外，一篇关于基于动态光学相干断层扫描评估人类肺泡类器官的合著论文发表在《生物医学光学快报》上。我们提出的显微镜的进一步发展也在2022财年进行。它包括允许我们的显微镜的纵向成像能力。这已经完成了整合培养室与显微镜，以允许模拟组织活动成像在培养过程中。目前，培养室的整合是成功的。通过培养室窗口和孔板盖成功获得了样品的D-OCT图像。它将允许将来对肿瘤球体和新鲜离体组织进行纵向药物反应评估。

In FY 2022, the proposed OCT microscope has been successfully used for the anti-cancer drug investigations of the tumor spheroid. Three anti-cancer drugs were applied to human breast cancer (MCF-7) spheroids and the dynamic OCT microscopic images revealed totally different response patterns among the applied drugs. In addition, the dynamic OCT patterns were consistent with the standard fluorescence microscopic images. The results may proof the feasibility of our proposed to perform anti-cancer drug investigations.The above mentioned results have been published as an oral and poster presentations in an international conference (Photonics West 2023, BiOS conference) and a journal paper is now under writing. Several co-authored work were published in the same conference. In addition, a co-authored paper about dynamic optical coherence tomography based evaluation of human derived alveolar organoids has been published in Biomedical Optics Express journal.Further development of our proposed microscope has been also performed during FY 2022. It includes allowing the longitudinal imaging ability of our microscope. This has been performed by integrating a cultivation chamber with the microscope to allow the simulations tissue activity imaging during cultivation. The cultivation chamber integration was successful so far. And we successfully obtained the D-OCT images though the cultivation chamber window and the cap of the well plate containing the samples. It will allow future longitudinal drug response evaluation of tumor spheroids and fresh ex-vivo tissue.