Study of the effects of alpha2 adrenoceptor on the development of epileptogenesis in immature rats

α2肾上腺素受体对幼鼠癫痫发生发展影响的研究

• 批准号: 09670990
• 负责人: YOSHIOKA Shin-ichi
• 金额: $ 2.11万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670990/
• 关键词: Epileptogenesis; Alpha 2 adrenoceptor; Amygdaloid kindling; Immature period; l-Histidine; Droxydopa; Rat; 幼若; 発達; α2アドレナリン受容体

The imbalance in inhibitory and excitatory neurotransmitters plays an important role in the development of epileptic seizures in human and animal models. Central noradrenergic systems are reported to suppress the development of kindling. However, noradrenergic systems are immature in postnatal period compared with in adult period. Therefore, to elucidate the effect of alpha-2 adrenoceptor on the development of epileptogenesis in immature period, we investigated the effects on the development of amygdaloid kindling in various aged rats (postnatal 14 days, 21 days, 28 days, and 70 days).1. Alpha-2 agonist, clonidine, suppressed the development of kindling in 28-day-old young rats as well as in adult rats. However, the development of kindling was facilitated by clonidine in 14-day-old pup rats.2. Facilitative effect of development of kindling by clonidine in 14-day-old pup rats was blocked with combined administration of clonidine and alpha-2 antagonist such as yohimbine, idazoxan or rauwolscine.3. Noradrenaline precursor droxydopa did not affect the development of kindling in 14-or 21-day-old rats.4. Histamne precursor 1-histidine caused inhibitory effects on the development of kindling in both adult and 14-, 21-day-old pup rats.The present pharmacological studies indicate that the effects of alpha-2 adrenoceptors on the development of amygdaloid kindling are age specific.

抑制性和兴奋性神经递质的失衡在人类和动物模型中癫痫发作的发展中起着重要作用。据报道，中央去甲肾上腺素能系统抑制点燃的发展。然而，与成人期相比，去肾上腺素能系统在产后时期不成熟。因此，为了阐明α-2肾上腺素受体对未成熟时期癫痫发生发展的影响，我们研究了对各种老年大鼠杏仁核生物发展的影响（产后14天，21天28天和70天）.1。 Alpha-2激动剂（可乐定）抑制了28天大的年轻大鼠和成年大鼠的点燃发展。然而，可乐定在14天大的幼犬中促进了点燃的发育。2。可乐定大鼠中可乐定生物发育开发的促进作用被可乐定和α-2拮抗剂（例如Yohimbine，idazoxan或rauwolscine.3）阻塞。3。去甲肾上腺素的前体曲氧化无影响不影响14或21天大的大鼠的点燃的发展。4。病变前体1-硫磺对成人和14天，21天大的幼虫大鼠的点燃发展产生抑制作用。当前的药理学研究表明，α-2肾上腺素受体对杏仁糖点燃的发展的影响是年龄特异性的。

项目成果

Shin-ichi Yoshioka: "Effects of histamine precursor l-histidine on the development of amygdaloid kindling in developing rats" J.Jpn.Epil.Soc. 17. 66 (1999)

Shin-ichi Yoshioka：“组胺前体 L-组氨酸对发育中大鼠杏仁核点火的影响”J.Jpn.Epil.Soc。

Seiji Katayama: "The effects of noradrenergic alpha-2 agonist clonidine on the development of amygdaloid kindling in immature rats" J.Yonago Med.Ass.18. 103-115 (1997)

Seiji Katayama：“去甲肾上腺素能 α-2 激动剂可乐定对未成熟大鼠杏仁核点燃发育的影响”J.Yonago Med.Ass.18。

吉岡 伸一: "成長段階のラット扁桃核キンドリング形成過程に及ぼすクロニジンの影響" てんかん研究. 16・1. 55-56 (1998)

吉冈真一：“可乐定对生长阶段大鼠杏仁核点燃过程的影响”癫痫研究16・1（1998）。

Katayama, S.: "The α2-agonisit clonidine facilitates the development of amygdaloid kindling in infant rats" Electroencephalography and clinical Neurophysiology. 98・4. 52 (1996)

Katayama，S.：“α2-激动剂可乐定促进幼鼠杏仁核的发育”，脑电图和临床神经生理学 98・4（1996）。

片山征爾: "幼若ラット扁桃体キンドリング形成過程におけるα2作動薬クロニジンの影響" 米子医学雑誌. 48・2. 103-115 (1997)

Seiji Katayama：“α2 激动剂可乐定对幼鼠杏仁核形成过程的影响”米子医学杂志 48・2（1997）。