Study of effects of endocrine disrupters on seizure susceptibility of amygdaloid kindling in developing rats

我们研究了围产期暴露于双酚A(BPA)，一种内分泌干扰物对发育中的大鼠杏仁核点燃的影响。(1)从妊娠第12天（GD）至产后第10天（PND），大鼠口服BPA （10 mg/kg）。在PND 1、3、5、7、9大鼠皮下注射苯甲酸雌二醇（Estradiol benzoate， EB） 20 μg/g。BPA处理显著增加了PND 14上幼鼠达到第5阶段癫痫发作（点燃率）的刺激次数，而降低了PND 70上的点燃率。然而，EB处理降低了PND 14和70的点燃率。(2)在不同组雄性大鼠杏仁核植入双极电极后，通过每日腹腔注射BPA（1、4、10 mg/kg）或EB(10、50、100 μg/kg)，观察BPA和EB对电引燃获得的影响。双酚a对其未产生显著影响，但50 μg/kg剂量的EB显著降低了其点燃率。(3)在完全点燃雄性大鼠后，分别给予大剂量BPA（1、2、10 mg/kg）或EB(10、30、100 μg/kg)，记录不同时间（30、90 min）与BPA或EB点燃前的点燃参数，如癫痫发作阶段、放电后持续时间（ADD）等。而30 μg/kg剂量的EB可显著增加add（作用时间为90min）。(4)从妊娠第12天（GD）至产后第10天（PND），大鼠口服BPA （10 mg/kg）。在PND 1、3、5、7、9大鼠皮下注射苯甲酸雌二醇（Estradiol benzoate， EB） 20 μg/g。双酚a或EB处理的大鼠在PND 10和70的空地上进行5分钟的测试。BPA没有对行为产生任何影响。然而，EB治疗降低了PND 70的活性。此外，BPA和EB处理在PND 10、30和60时显著降低了雄性和雌性大鼠的体重。此外，EB处理显著降低了PND 80雄性的睾丸重量，但BPA对睾丸重量没有影响。这些结果表明，围产期暴露于内分泌干扰物可改变大鼠杏仁核体点燃的癫痫易感性，但BPA的影响与EB不同。少

We investigated the effects of perinatal exposure to bisphenol A(BPA), an endocrine disrupter on the development of amygdaloid kindling in developing rats.(1)BPA (10 mg/kg) was administered orally to mother rats from gestation day(GD) 12 through postnatal day(PND) 10. Estradiol benzoate(EB) (20 μg/g) was subcutaneously injected to rats on PND 1,3,5,7 and 9. BPA treatment increased significantly the number of stimulations to reach the first stage 5 seizure (kindling rate) in pups on PND 14, while decreased the kindling rate on PND 70. However, EB treatments decreased the kindling rate on both PND 14 and 70.(2)After implantation of bipolar electrodes in amygdala, effects of BPA and EB on electrical kindling acquisition were investigated by daily intraperitoneal injection of BPA (1,4,10 mg/kg) or EB (10,50,100 μg/kg) in different groups of male rats. BPA failed to produce any significant effect, but EB at dose of 50 μg/kg decreased significantly the kindling rate.(3)After the completion o … More f amygdaloid kindling, fully kindled male rats were treated with voirous doses of BPA (1,2,10 mg/kg) or EB (10,30,100 μg/kg), and kindled seizure parameters such as seizure stage, after-discharge duration(ADD) were recorded at various times (30 and 90 min) compared with the kindling parameters before treatment of BPA or EB. BPA failed to produce any effect, while 30- μg/kg dose of EB produced a significant increment of ADDs (after 90 min).(4)BPA (10 mg/kg) was administered orally to mother rats from gestation day(GD) 12 through postnatal day(PND) 10. Estradiol benzoate(EB)(20 μg/g) was subcutaneously injected to rats on PND 1,3,5,7 and 9. Rats treated with BPA or EB were tested in the open-field for 5 min on PND 10 and 70. BPA failed to produce any effect on the behavior. However, EB treatments reduced activity on PND 70. In addition, both BPA and EB treatments significantly reduced body weight of both male and female rats on PND 10,30, and 60. In addition, EB treatments reduced significantly testis weight in males on PND 80, but BPA did not affect the testis weight.These results suggest that perinatal exposure to endocrine disrupters modified the seizure susceptibility of amygdaloid kindling in rats, while the effects of BPA were different from those of EB. Less