Study of the mechanisms of the persistence seizure susceptibility acquired in the developing periods in amygdaloid kindled rats

杏仁核激发大鼠发育期持续性癫痫易感性机制研究

基本信息

批准号：
13671005
负责人：
YOSHIOKA Shin-ichi
金额：
$ 2.3万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671005/
关键词：
amygdaloid kindling seizure susceptibility epileptogenesis monosodium glutamate methotrexate immature re-kindling rat

项目摘要

We investigated whether the primary and secondary epileptogenesis acquired in developing periods persists into adulthood using amygdaloid kindling rats. When the initial kindling was established at age 40 and 70 days, the number of stimulations required to induce the first stage 5 seizure at the contralateral as well as ipsilateral sites was significantly fewer in kindled rats than in non-stimulated controls. When the initial kindling was established at age 14 days the number of stimulations required to induce the first stage 5 seizure at neither ipsilateral or contralateral site in kindled rats was not significantly different from that in non-stimulated controls. When the initial kindling was established at age 28 days, the facilitative effect was observed at the ipsilateral site, but not at the contralateral site. These results suggest that both the primary and secondary epileptogenesis acquired at age 14 days is diminished after maturation, and the persistence of seizure susceptibility acquired in the developing periods is weaker in the primary than secondary site.To clarify the mechanisms of the persistence of seizure susceptibility, we (MTX) treatments in rats modify the development of amygdaloid kindling in immature period and the persistence of seizure susceptibility during development. Both MSG and MTX treatments decreased significantly the number of stimulations required to reach the first stage 5 seizure in pups at age 14 days. When the rats were rekindled at age 70 days, the number of stimulations required to reinduce the first stage 5 seizure was significantly lower in rats treated with both MSG and MTX than that in control rats. These results suggest that the susceptibility to kindling-induced seizure in immature rats is enhanced with both MSG and MTX treatments, and that the enhanced seizure susceptibility acquired in immature period persists into maturity.

我们调查了使用杏仁核生物的大鼠在发育时期获得的主要和继发性癫痫发生是否持续到成年。当最初的点燃是在40天和70天内建立的时，在对比的大鼠中诱导第一阶段5癫痫发作所需的刺激数量明显少于未刺激的对照组。当在14天内确定初始点燃时，在生物的大鼠中诱导第一阶段5癫痫发作所需的刺激次数与未刺激的对照组中均未显着差异。当最初的点燃在28天内建立时，在同侧位置观察到促进作用，但在对侧位置却没有观察到促进作用。 These results suggest that both the primary and secondary epileptogenesis acquired at age 14 days is diminished after maturation, and the persistence of seizure susceptibility acquired in the developing periods is weaker in the primary than secondary site.To clarify the mechanisms of the persistence of seizure susceptibility, we (MTX) treatments in rats modify the development of amygdaloid kindling in immature period and the persistence of seizure开发过程中的敏感性。 MSG和MTX治疗都显着减少了14天以上幼崽中幼崽第5阶段癫痫发作所需的刺激次数。当大鼠在70天后重新点燃大鼠时，用MSG和MTX治疗的大鼠诱导第一阶段5癫痫发作所需的刺激数量明显低于对照大鼠的刺激。这些结果表明，通过MSG和MTX处理，增强了对未成熟大鼠发射诱导的癫痫发作的敏感性，并且在未成熟时期内获得的增强的癫痫发作易感性持续成熟。