Investigation of stress response in osteoblasts

成骨细胞应激反应的研究

• 批准号: 09671041
• 负责人: KOZAWA Osamu
• 金额: $ 1.41万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671041/
• 关键词: heat shock protein; osteoblast; protein kinase C; mitogen-activated protein kinase; prostaglandin; endothelin; sphingosine 1-phosphate

We investigated the effects of various physiological agonists on heat shock protein (HSP)27 induction in osteoblasts and the intracellular signaling system. We obtained the results as follows :1. Prostaglandin F2alpha stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by prostaglandin F2alpha. In addition, p42/p44 mitogen-activated protein (MAP) kinase plays at a downstream from protein kinase C.2. Endothelin-1 stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by endothelin-1. In addition, p38MAP kinase plays at a downstream from protein kinase C.3. Sphingosine 1-phosphate, a metabolite of sphingomyelin, stimulates HSP27 induction in osteoblast-like MC3T3-E1 cells. p38MAP kinase is involved in the HSP27 induction by Sphingosine 1-phosphate.

我们研究了各种生理激动剂对成骨细胞中热休克蛋白（HSP）27诱导和细胞内信号系统的影响。主要研究结果如下：1.前列腺素F2 α刺激成骨样MC 3 T3-E1细胞中的HSP 27诱导前列腺素F2 α对HSP 27的诱导作用与蛋白激酶C的激活有关。此外，p42/p44丝裂原活化蛋白（MAP）激酶在蛋白激酶C.2的下游起作用。内皮素-1刺激成骨样MC 3 T3-E1细胞中HSP 27的诱导蛋白激酶C的激活参与了内皮素-1诱导HSP 27的表达。此外，p38 MAP激酶在蛋白激酶C的下游起作用。鞘磷脂的代谢产物1-磷酸鞘氨醇刺激成骨样MC 3 T3-E1细胞中HSP 27的诱导。p38 MAP激酶参与1-磷酸鞘氨醇对HSP 27的诱导。

项目成果

Kozawa, O.: "Sphingosine modulates interleukin-6 synthesis in osteoblasts." J.Cell.Biochem.70. 338-345 (1998)

Kozawa, O.：“鞘氨醇调节成骨细胞中白细胞介素 6 的合成。”

Kozawa Osamu: "Interleukin-6 synthesis induced by prostaglandin E2 : cross-talk regulation by protein kinase C." Bone. 22. 355-360 (1998)

Kozawa Osamu：“前列腺素 E2 诱导的白细胞介素 6 合成：蛋白激酶 C 的串扰调节。”

Osamu Kozawa: "Tumor necrosis factor-α autoregulates interleukin-6 synthesis via activation of protein kinase C" The Journal of Biological chemistry. 272. 25099-25104 (1997)

Osamu Kozawa：“肿瘤坏死因子-α 通过激活蛋白激酶 C 自动调节白细胞介素 6 的合成”《生物化学杂志》272. 25099-25104 (1997)。

Kozawa, O.: "Thrombin regulates interleukin-6 synthesis through phosphatidylcholine hydrolysis by phospholipase D in osteoblasts." Arch.Biochem.Biophys.345. 10-15 (1997)

Kozawa, O.：“凝血酶通过成骨细胞中磷脂酶 D 水解磷脂酰胆碱来调节白细胞介素 6 的合成。”

Kozawa, O.: "Tumor necrosis factor-α autoregulates interleukin-6 synthesis via activation of protein kinase C." J.Biol.Chem.272. 25099-25104 (1997)

Kozawa, O.：“肿瘤坏死因子-α 通过蛋白激酶 C 的激活自动调节白细胞介素 6 的合成。J.Biol.Chem.272 (1997)。