Investigation of stress response in osteoblasts

我们研究了各种生理激动剂对成骨细胞热休克蛋白（HSP）27诱导和细胞内信号系统的影响。我们得到的结果如下：1。前列腺素F2alpha刺激成骨细胞样MC3T3-El细胞HSP27的诱导。蛋白激酶C的活化参与了前列腺素F2alpha诱导HSP27的过程。此外，p42/p44有丝分裂原活化蛋白（MAP）激酶在蛋白激酶C.2的下游起作用。内皮素-1刺激成骨细胞样MC3T3-El细胞HSP27的诱导。蛋白激酶C的活化参与了内皮素-1诱导HSP27的过程。此外，p38MAP激酶在蛋白激酶C.3的下游发挥作用。鞘磷脂的代谢物鞘磷脂1-磷酸刺激成骨细胞样MC3T3-E1细胞HSP27的诱导。p38MAP激酶参与鞘氨醇1-磷酸诱导HSP27。

We investigated the effects of various physiological agonists on heat shock protein (HSP)27 induction in osteoblasts and the intracellular signaling system. We obtained the results as follows :1. Prostaglandin F2alpha stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by prostaglandin F2alpha. In addition, p42/p44 mitogen-activated protein (MAP) kinase plays at a downstream from protein kinase C.2. Endothelin-1 stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by endothelin-1. In addition, p38MAP kinase plays at a downstream from protein kinase C.3. Sphingosine 1-phosphate, a metabolite of sphingomyelin, stimulates HSP27 induction in osteoblast-like MC3T3-E1 cells. p38MAP kinase is involved in the HSP27 induction by Sphingosine 1-phosphate.