Investigation of p20, a heat shock protein, in platelet function

研究 p20（一种热休克蛋白）在血小板功能中的作用

• 批准号: 11838005
• 负责人: KOZAWA Osamu
• 金额: $ 2.3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11838005/
• 关键词: platelet; heat shock protein; vascular smooth muscle; aggregation; 凝集

We investigated the effects of p20, a low molecular weight heat shock protein, on platelet aggregation and the mechanism. We obtained the results as follows:1. p38 mitogen-activated protein kinase is required for vasopressin-stimulated HSP27 induction in vascular smooth muscle cells, and the activation of p38 mitogen-activated protein kinase is dependent on protein kinase C.2. αB-crystallin, which is discharged from vessel walls in response to endothelial injury, acts intercellularly as a regulator of platelet function. Moreover, these findings could be a part of new concept of antiplatelet therapy in the treatment of cardiovascular diseases.

我们研究了低分子量热休克蛋白p20对血小板聚集的影响及其机制。主要研究结果如下：1. p38丝裂原活化蛋白激酶是加压素刺激的血管平滑肌细胞中HSP 27诱导所必需的，并且p38丝裂原活化蛋白激酶的活化依赖于蛋白激酶C。α B-晶状体蛋白，响应内皮损伤而从血管壁排出，作为血小板功能的调节剂在细胞间起作用。这些发现可能为心血管疾病抗血小板治疗的新概念提供一部分。

项目成果

Kozawa, O.: "Sphingosine 1-phosphate regulates heat shock protein 27 induction by a p38 MAP-kinase dependent mechanism in aortic smooth muscle cells"Exp. Cell Res.. 250. 376-380 (1999)

Kozawa, O.：“1-磷酸鞘氨醇通过主动脉平滑肌细胞中的 p38 MAP 激酶依赖性机制调节热休克蛋白 27 的诱导”Exp。

Osamu Kozawa: "αB-crystallin, a low molecular weight heat shock protein, acts of a requlator …"Cell Stress Chaperones. 6. 21-28 (2001)

Osamu Kozawa：“αB-晶状体蛋白，一种低分子量热休克蛋白，具有调节剂的作用......”细胞应激伴侣。 6. 21-28 (2001)

Kaida,T.: "Vasopressin stimulates the induction of heat shock protein 27 and a B-crystallin via protein kinase C activation in vascular smooth muscle cells"Exp. Cell Res.. 246. 327-337 (1999)

Kaida,T.：“加压素通过血管平滑肌细胞中的蛋白激酶 C 激活刺激热休克蛋白 27 和 B-晶状体蛋白的诱导”Exp。

Takehiro Kaida: "Vasopressin stimulates the induction of heat shock protein 27 and αβ-crystallin via protein kinase C ---"Experimental Cell Research. 246. 327-337 (1999)

Takehiro Kaida：“加压素通过蛋白激酶 C 刺激热休克蛋白 27 和 αβ-晶状体蛋白的诱导 ---”实验细胞研究 246. 327-337 (1999)。

Osamu Kozawa: "Sphingosine 1-phosphete regulates heat shock protein 27 induction by a p38 MAP-Kinase-dependent mechanism …"Experimental Cell Research. 250. 376-380 (1999)

Osamu Kozawa：“1-磷酸鞘氨醇通过 p38 MAP 激酶依赖性机制调节热休克蛋白 27 的诱导……”实验细胞研究 250. 376-380 (1999)。