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An analysis of collateral formation and vascular smooth muscle cell migration

侧支形成和血管平滑肌细胞迁移的分析

基本信息

批准号：
09671214
负责人：
YASUHARA Hiroshi
金额：
$ 2.05万
依托单位：
Teikyo University (1998-1999)The University of Tokyo (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671214/
关键词：
Smooth muscle cell Endothelial cell migration collatenal circulation Shear stress platelet PDGF

项目摘要

Migration and proliferation of smooth muscle cells (SMC) are the main mechanisms of development of collateral formation to ischemic organs. However, it has not yet been elucidated how mechanical stress is involved in this process. We investigated smooth muscle cell mitogenic activity induced by shear-loaded platelets and endothelial cells. METHODS: Experimental design: in vitro experimental study. We devised four types of conditioned media; supernatant of mixed culture of platelets and endothelial cell (ST), supernatant of shear-loaded mixed culture of platelets and endothelial cell (SH), ST medium neutralised with anti-PDGF antibody (ST+), and SH medium neutralised with anti-PDGF antibody (SH+). Smooth muscle cells were cultured in each conditioned medium, and their spreading activity was determined under a microscope. RESULTS: Smooth muscle cells spreading activity in the SH group was significantly greater than that in the ST group. Their spreading activity was suppressed by anti-PDGF antibody under shear conditions (SH+), but it was not by anti-PDGF antibody under static conditions (ST+). CONCLUSIONS: Our results demonstrate that platelet-derived growth factor is produced by shear-loaded platelets and endothelial cells, and local mechanical forces may play an important role in cardiovascular disease.

平滑肌细胞的迁移和增殖是缺血器官侧支形成的主要机制。然而，尚未阐明机械应力如何参与这一过程。我们研究了剪切负荷血小板和内皮细胞诱导的平滑肌细胞促有丝分裂活性。方法：实验设计：体外实验研究。我们设计了四种类型的条件培养基：血小板和内皮细胞的混合培养物的上清液（ST），血小板和内皮细胞的剪切加载的混合培养物的上清液（SH），用抗PDGF抗体中和的ST培养基（ST+），和用抗PDGF抗体中和的SH培养基（SH+）。在每种条件培养基中培养平滑肌细胞，并在显微镜下测定其铺展活性。结果：SH组平滑肌细胞伸展活性明显高于ST组。在剪切条件下（SH+），抗PDGF抗体可抑制它们的铺展活性，但在静态条件下（ST+），抗PDGF抗体对它们的铺展活性无抑制作用。结论：我们的研究结果表明，血小板衍生生长因子是由剪切加载的血小板和内皮细胞产生的，局部机械力可能在心血管疾病中发挥重要作用。