Repression of plant genes by TFL2/LHP1 and Polycomb Group Proteins

植物和动物中基因表达的表观遗传抑制是由染色质修饰介导的，例如H3的9或27位赖氨酸残基的甲基化。使用芯片方法表明，与基于序列同源性的预测相反，拟南芥蛋白TFl2/LHP1是动物HP1的近亲，通过H3K27me3抑制许多调控基因的转录。为了更好地了解TFl2/LHP1在植物中的作用，我们建议鉴定TFl2/LHP1的相互作用伙伴，并阐明TFl2/LHP1在H3K27me3相关靶基因的转录抑制中所起的特定功能。简介：H3：组蛋白3；CHIP-CHIP：染色质免疫沉淀杂交到DNA微阵列；TFL2：末端花2；LHP1：LIKEHETEROChROIN Protein 1；HP1：异染色质蛋白1；H3K27me3：组蛋白3-赖氨酸27三甲基；

Epigenetic repression of gene expression in plants and animals is mediated by chromatin modifications such as methylation of lysine residues of H3 at position 9 or 27. By using a ChIP-chip approach showed that contrary to predictions based on sequence homology, the Arabidopsis protein TFL2/LHP1, a relative of animal HP1, plays a role in repressing transcription of many regulatory genes via H3K27me3. To better understand the role of TFL2/LHP1 in plants, we propose to identify interaction partners of TFL2/LHP1 and elucidate the specific function that TFL2/LHP1 plays in the transcriptional repression of a subset of H3K27me3-associated target genes.Abbreviations in summary: H3: HISTONE 3; ChIP-chip: Chromatin immunoprecipitation hybridized to DNA microarray; TFL2: TERMINAL FLOWER 2; LHP1: LIKEHETEROCHROMATIN PROTEIN 1; HP1: HETEROCHROMATIN PROTEIN 1; H3K27me3: HISTONE 3-lysine 27 tri-methyl;