Regulation of immune response in hole body and intestinal mucosa by oral administration of staphylococcal enterotoxin B (SEB)

我们已经决定了oral SEB administration prevented the onset of DSS induced colitis，clonal deletion designated by Vβ 8t -cells. We evaluated the effect of oral SEBadministration on TCR α KO mice. (Experiment 1) Simultaneous administration of SEB ameliorated DSSinduced colitis on TCR α KO mice，following prolonged survival time. Although serum Ig M of mice administered DSS increased more thanthat of control，the cell fractions of CD4 - D1+ D1TCRVβ - D1dim D1T-cells and CD4 - D1+ D1TCRVβ - D1dim D1T-cells（Experiment 2） Although TCR α KO mice have （Experiment 2）been given SEB for 9 weeks，the incidence of colitis did not decrease and the cell fractions ofCD4 - D1+ D1TCRVβ - D1dim - D1T-cells also did not change. It was suggested thatCD4 - D1+ D1TCRVβ - D1dim - D1T-cell may influence the onset of the colitis on TCR α KO mice. We在DSS induced colitis of TCR α KO mice. Therefore这是suggested that TCRα D1- e e D1β - e D1 cells which express the Vβ region corresponding SEB mayplay an important role on the onset of DSS induced colitis on TCR α KO mice. However，it was difficult to induce oral tolerance by SEB under condition of continuing local inflammationsuch as colitis of TCR α KO mice。

We have been elucidated that oral SEB administration prevented the onset of DSS induced colitis, and influence of clonal deletion designated by Vβ8T -cells. We evaluated the effect of oral SEB administration on TCR α KO mice. (Experiment 1) Simultaneous administration of SEB ameliorated DSS induced colitis on TCR α KO mice, following prolonged survival time. Although serum Ig M of mice administered DSS increased more than that of control, the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells and CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells did not change with or without SEB oral administration. (Experiment 2) Although TCR α KO mice have been given SEB for 9 weeks, the incidence of colitis did not decrease and the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells also did not change. It was suggested that CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cell may influence the onset of the colitis on TCR α KO mice. We elucidated that preventive effect of SEB on DSS induced colitis of TCR α KO mice. Therefore, it is suggested that TCRαィイD1-ィエD1βィイD1+ィエD1 cells which express the Vβ region corresponding SEB may play an important role on the onset of DSS induced colitis on TCR α KO mice. However, it was difficult to induce oral tolerance by SEB under condition of continuing local inflammation such as colitis of TCR α KO mice.