Analysis of Surfactant-associated Proteins in injured lungs

损伤肺中表面活性剂相关蛋白的分析

• 批准号: 09670626
• 负责人: UEDA Takashi
• 金额: $ 1.73万
• 依托单位: DOKKYO UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670626/
• 关键词: Surfactant; Surfactant Protein A; injury lung; Bronchial Asthma; Chemotactis activity; Apoptosis; Eosinophil; Cytokine; 気道上皮; GM-CSF; 脱顆粒

We investigated suffactant system in acute lung injury and asthmatic lungs. We found that suffactant protein A (SP-A) could stimulate chemotaxis of bronchial epithelial cells, suggesting that SP-A might play a role in lung repair following injury as a chernoattractant. We also reported that altered surfactant system and metabolism in rat lungs injured by LPS instillation. We also reported compositional and functional changes of surfactant in a guinea pig model of chronic asthma and increased levels of SP-A in the airway in patients with bronchial asthma. The interactions between surfactant and eosinophils were also studied. We demonstrated surfactant could induce apoptosis of eosinophils, surpress IL-8production by eosinophils and inhibit ECP degranulation. All these new findings must open a new array to study the involvement of surfactant in the pathogenesis of bronchial asthma and acute lung injury.

本研究探讨了急性肺损伤和哮喘肺组织中的氧化物系统。我们发现，窒息蛋白A（SP-A）可以刺激支气管上皮细胞的趋化性，提示SP-A可能作为一种趋化因子在损伤后的肺修复中发挥作用。我们还报道了LPS灌注损伤大鼠肺表面活性物质系统和代谢的改变。我们还报道了豚鼠慢性哮喘模型中表面活性物质的组成和功能变化以及支气管哮喘患者气道中SP-A水平的增加。表面活性剂和嗜酸性粒细胞之间的相互作用也进行了研究。结果表明，表面活性剂可诱导嗜酸性粒细胞凋亡，抑制嗜酸性粒细胞产生IL-8，抑制ECP脱颗粒。这些新发现为研究肺表面活性物质在支气管哮喘和急性肺损伤发病机制中的作用开辟了新的思路。

项目成果

Cheng G: "Suppreive effects of SP-A on couomyin induced IL-8 production and release by eosinophils" Int Arch Allergy Iurnruvol. 117. 59-62 (1998)

Cheng G：“SP-A 对香豆素诱导的嗜酸性粒细胞 IL-8 产生和释放的抑制作用”Int Arch Allergy Iurnruvol。

Kinjyo S,T.Ueda et al.: "Surfactant can induce eosinophil apoptosis. Int Arch Allergy Immunol" Int Arch Allergy Immunol. (in press.).

Kinjyo S,T.Ueda 等人：“表面活性剂可以诱导嗜酸性粒细胞凋亡。Int Arch Allergy Nutrition”Int Arch Allergy Nutrition。

T.Ueda et al: "Effect of aging on surfactant forms in rats." Eur Resp J. (in press).

T.Ueda 等人：“衰老对大鼠表面活性剂形式的影响”。

上田 隆: "気管支喘息患者における肺サーファクタントの検討" アレルギー. 46(8.9). 912- (1997)

Takashi Ueda：“支气管哮喘患者肺表面活性物质的研究”46(8.9)。

金城 俊一: "肺サーファクタントの好酸球生存延長に与える効果" アレルギー. 46(8.9). 908- (1997)

Shunichi Kinjo：“肺表面活性物质对延长嗜酸性粒细胞存活的影响”过敏症46(8.9)。