Isolation and functional expression of novel putative carbon dioxide(CO_2) receptors in respiratory epithelium.

呼吸道上皮中新型假定二氧化碳（CO_2）受体的分离和功能表达。

• 批准号: 21590238
• 负责人: UEDA Takashi
• 金额: $ 3万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590238/
• 关键词: 受容体; 細胞内シグナル伝達; 化学感覚; 化学受容器; シグナル伝達; 呼吸器系; 上皮細胞; 嗅覚系CO_2受容器; 嗅覚系CO2受容機; 嗅覚系CO2受容機構

Mouse nasal epithelial cells partially exhibited CO_2-induced intracellular calcium mobilization. These cells did not respond to the ligands that activate proton-sensitive cation channels, such as transient receptor potential vanilloid 1(TRPV1), acid-sensing ion channel(ASIC) 1a, ASIC1b, ASIC2a, ASIC2b and ASIC3.Since the nasal epithelial cells responded to NaHCO_3 solution rather than acidic pH solution, a mechanism for detection of HCO_3-may be involved in nasal CO_2 perception.

小鼠鼻粘膜上皮细胞部分表现出CO_2诱导的细胞内钙动员。这些细胞对激活质子敏感阳离子通道的配体如瞬时受体电位香草酸1（TRPV 1）、酸敏感离子通道（ASIC）1a、ASIC 1b、ASIC 2a、ASIC 2b和ASIC 3均无反应。由于鼻上皮细胞对NaHCO_3溶液而非酸性pH溶液有反应，因此HCO_3-的检测机制可能与鼻CO_2感受有关。

项目成果

Acid inhibits TRPV4-mediated Ca^2+influx in mouse esophageal epithelial cells

酸抑制小鼠食管上皮细胞中 TRPV4 介导的 Ca^2 内流

• DOI: 10.1111/j.1365-2982.2011.01767.x
• 发表时间: 2011
• 期刊: Neurogastroenterol Motil.
• 作者: Shikano M;Ueda T;Kamiya T;Ishida Y;Yamada T;Mizushima T;Shimura T;Mizoshita T;Tanida S;Kataoka H;Shimada S;Ugawa S;Joh T
• 通讯作者: Joh T

Development of generic calcium imaging assay for monitoring Gi-coupled receptors and G protein interaction

开发用于监测 Gi 偶联受体和 G 蛋白相互作用的通用钙成像测定法

• 发表时间: 2009
• 期刊: J Biomol Screen. 14
• 作者: Ueda T;Ugawa S;Ishida Y;Hondoh A;Shimada S.
• 通讯作者: Shimada S.

Geissoschizine methyl ether has third-generation antipsychotic-like actions at the dopamine and serotonin receptors

• DOI: 10.1016/j.ejphar.2011.09.007
• 发表时间: 2011-12-05
• 期刊: EUROPEAN JOURNAL OF PHARMACOLOGY
• 影响因子: 5
• 作者: Ueda, Takashi;Ugawa, Shinya;Shimada, Shoichi
• 通讯作者: Shimada, Shoichi

P2X2- and P2X3-Positive Fibers in Fungiform Papillae Originate From the Chorda Tympani but Not the Trigeminal Nerve in Rats and Mice

• DOI: 10.1002/cne.22000
• 发表时间: 2009-05-10
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Ishida, Yusuke;Ugawa, Shinya;Shimada, Shoichi
• 通讯作者: Shimada, Shoichi

Distinct expression of cold receptors (TRPM8 and TRPA1) in the rat nodose-petrosal ganglion complex

• DOI: 10.1016/j.brainres.2010.01.016
• 发表时间: 2010-03-10
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Hondoh, Aki;Ishida, Yusuke;Shimada, Shoichi
• 通讯作者: Shimada, Shoichi