The Study on the Mechanism of Ischemic Preconditioning of the Myocardium
心肌缺血预适应机制的研究
基本信息
- 批准号:09670738
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
ATP-sensitive K channel (KィイD2ATPィエD2 channel) have been reported to play a major role in ischemic preconditioning. The present study was designed to clarify the effect of mitochondrial KィイD2ATPィエD2 channel opener on the hypoxia-reperfusion injury. Ventricular myocytes isolated from Sprague-Dawley rat were studied. Cardiomyocytes were superfused with anoxic glucose-free Tyrode solution, which was equilibrated with 99.99% nitrogen under positive pressure, for 15 minutes followed by normoxic Tyrode solution for 40 minutes. Diazoxide (30 μM), a specific mitochondrial KィイD2ATPィエD2 channel opener was administered to the diazoxide group (D group) 5 minutes prior to anoxic perfusion until the end of anoxic perfusion. Intracellular pH measured by BCECF and the shape of myocytes were recorded by microscopic fluorometric system ARGUS 200 (Hamamatsu Photonics, Hamamatsu, Japan). Rod-shaped cell was considered to be alive. The viability of cardiomyocytes at the end of anoxia-reoxygenation perfusion was significantly higher in D group compared with control group (59.3±10.6 vs 22.0±13.3% ; n=8,6 rats ; <0.05). The pH of myocytes was similar between two groups at the end of anoxic perfusion. Most of the killed cells of the control group showed round cells but the half of the killed cell of D group showed rigor contracture (control group : round cells 62.3%, rigor contracture 10.7% ; D group : round cells 20.3%, rigor contracture 19.5%). When isolated cardiomyocytes were made skinned by saponin after washing by EGTA-containing solution, and suspended in ATP-depleted solution, myocytes became rigor contracture. Mitochondrial KィイD2ATPィエD2 channel opener showed the protective effect on the anoxia-reoxygenation injury of the isolated cardiomyocyte of rat without any change of intracellular pH. This protective effect might be due to reducing Ca overload during anoxia-reoxygenation perfusion.
三磷酸腺苷敏感钾通道(KィイD2ATPィエD2通道)在缺血预适应中发挥重要作用。本研究旨在阐明线粒体KィイD2ATPィエD2通道开放剂在缺氧再灌注损伤中的作用。对分离的SD大鼠心室肌细胞进行了研究。心肌细胞用99.99%氮气在正压下平衡的缺氧无葡萄糖泰氏液灌流15min,然后常氧泰氏液灌流40min。二氮嗪组(D组)于缺氧前5min给予线粒体特异性KィイD2ATPィエD2通道开放剂二氮嗪(30μM),直至缺氧灌流结束。用BCECF法测定细胞内pH,用Argus 200显微荧光测量系统记录心肌细胞的形态。杆状细胞被认为是活的。缺氧-复氧灌流结束时,D组心肌细胞存活率显著高于对照组(59.3±10.6vs22.0±13.3%;n=8,6;t;0.05)。缺氧灌流结束时,两组心肌细胞的pH值相近。对照组杀伤细胞多呈圆形,D组半数细胞呈僵硬挛缩(对照组:圆形细胞62.3%,僵直挛缩10.7%;D组:圆形细胞20.3%,僵直挛缩19.5%)。将分离的心肌细胞用含EGTA的溶液清洗后用皂苷去皮,悬浮在去ATP溶液中,心肌细胞发生僵硬的收缩。线粒体K-ィイ-D2ATP-ィエ-D2通道开放剂对大鼠心肌细胞缺氧-复氧损伤有保护作用,但对细胞内pH无明显影响。这种保护作用可能与减少缺氧-复氧灌流过程中的钙超载有关。
项目成果
期刊论文数量(0)
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Shiotani M、Kawabe T、Hano T、Baba A 等人:“尼卡地平减轻了离体微灌注兔子传入动脉的缺血/再灌注损伤”高血压杂志。
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Shiotani M., Kawabe T., Hano T., Baba A., et al.: "Nicardipine diminished ischemia/reperfusion injury on isolated microperfused rabbeit afferent arter arterioles."Journal of Hypertension. 16 (suppl2). S84 (1998)
Shiotani M.、Kawabe T.、Hano T.、Baba A. 等人:“尼卡地平可减轻离体微灌注兔传入动脉小动脉的缺血/再灌注损伤。”高血压杂志。
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Imanishi T.: "Effects of locally administered argatroban on restenosis after ballon angioplasty : experimental and clinical study" Clin Exp Pharmcol Physiol. 24. 800-806 (1997)
Imanishi T.:“局部施用阿加曲班对球囊血管成形术后再狭窄的影响:实验和临床研究”Clin Exp Pharmcol Physiol。
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Ueshima K., Baba A., et al.: "Clinical significance of 24-hour delayed thallium-201 imaging after exercise-redistribution imaging."Wakayama Medical Reports. 38 (1-4). 17-22 (1997)
Ueshima K.、Baba A. 等人:“运动再分布成像后 24 小时延迟铊 201 成像的临床意义。”和歌山医学报告。
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大森久司、羽野卓三、他: "高血圧自然発症ラットの副腎髄質からのエピネフリン放出増加に及ぼすカルモデュリン依存性プロテインキナーゼIIの関与" 心臓. 30(suppl2). 15-17 (1998)
Hisashi Omori、Takuzo Hano 等人:“钙调蛋白依赖性蛋白激酶 II 参与自发性高血压大鼠肾上腺髓质释放增加”Cardiac 30(增刊 2)。
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BABA Akira其他文献
Detection of Human Immunoglobulin G by Transmission Surface Plasmon Resonance Using the <i>In Situ</i> Gold Nanoparticle Growth Method
使用<i>原位</i>金纳米颗粒生长方法通过透射表面等离子共振检测人免疫球蛋白 G
- DOI:
10.1587/transele.2018omp0004 - 发表时间:
2019 - 期刊:
- 影响因子:0.5
- 作者:
JUAGWON Theerasak;LERTVACHIRAPAIBOON Chutiparn;SHINBO Kazunari;KATO Keizo;SRIKHIRIN Toemsak;OSOTCHAN Tanakorn;BABA Akira - 通讯作者:
BABA Akira
Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera
用于银纳米棱镜比色测定的光学传感平台及其在使用移动设备摄像头进行过氧化氢和葡萄糖检测中的应用
- DOI:
10.2116/analsci.18p412 - 发表时间:
2019 - 期刊:
- 影响因子:1.6
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LERTVACHIRAPAIBOON Chutiparn;MARUYAMA Takuya;BABA Akira;EKGASIT Sanong;SHINBO Kazunari;KATO Keizo - 通讯作者:
KATO Keizo
BABA Akira的其他文献
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{{ truncateString('BABA Akira', 18)}}的其他基金
Light Trapping via Multiple Plasmonic Structures and Their Applications to Organic Solar Cells
多重等离子体结构的光捕获及其在有机太阳能电池中的应用
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25390051 - 财政年份:2013
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Fabrication of Combined Plasmonic Organic Thin Films Solar Cells
组合等离子体有机薄膜太阳能电池的制造
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23760010 - 财政年份:2011
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$ 2.37万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The study of social model of the nobility in Meiji era, based in historical informatics
基于历史信息学的明治时代贵族社会模式研究
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21240023 - 财政年份:2009
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Grant-in-Aid for Scientific Research (A)
The dynamics of 《amour de soi》 in the aesthetics of pygmalionism in the 18^<th> century's France
《爱情》在18世纪法国皮格马利翁主义美学中的动态
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19520103 - 财政年份:2007
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Fabrication and Applications of Aligned Conducting Polymers-Carbon Nanotubes Hybrid Thin Films by Electropolymerization
电聚合定向导电聚合物-碳纳米管杂化薄膜的制备及应用
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19810005 - 财政年份:2007
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$ 2.37万 - 项目类别:
Grant-in-Aid for Young Scientists (Start-up)
Reconstructing Historical Culture and Natural Spectacle in our Country by Combined Materials of Volcano Afflicted Ground : Mainly North Kanto
结合火山灾区材料重建我国历史文化和自然景观:以北关东为主
- 批准号:
16089203 - 财政年份:2004
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
An Actual Study of"OIEBORI" by the Goldsmiths Gotou Family
金匠后藤家对“OIEBORI”的实际研究
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13610066 - 财政年份:2001
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research for Issue and Currency of Gold coin in the early modern Japanese Society
日本近代社会早期金币的发行与货币研究
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09630076 - 财政年份:1997
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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