Analysis of the healing of MRSA-infected cutaneous wounds prepared in hereditary diabetic mice

临床上众所周知，细菌感染延迟慢性皮肤溃疡的愈合。在这项研究中，我们成功地证明了遗传性糖尿病小鼠作为观察感染性伤口愈合过程的模型的实用性。通过将MRSA（耐甲氧西林金黄色葡萄球菌）施加到在雌性G57 BLKsJ db/db遗传性糖尿病小鼠的背部皮肤中产生的两个全层伤口来制备感染性慢性皮肤伤口。局部应用MRSA，然后用Cathereep膜覆盖伤口。贴敷后13天，通过测量上皮再生（%）、肉芽组织面积（mm^2）、毛细血管数量和细菌计数对伤口进行组织学评价。结果表明，糖尿病小鼠感染性溃疡和愈合不良的发生率高于正常小鼠，并有以下新的发现：（1）糖尿病小鼠创面覆盖时间长，表现较正常小鼠严重; ...更多信息 他打开伤口;（2）确定直径为3 mm的伤口是感染伤口的合适实验尺寸，因为较大的伤口在一个月的孵育期后不会闭合;（3）确定1 *10^6是最佳接种量;（4）建立MRSA感染平均需要4天;（5）MRSA感染的伤口在孵育13天后仍未愈合;（6）甲氧西林敏感株（MS）SA N315 P和MRSA N315 PZR对糖尿病小鼠感染性创面的形成具有相似的致病性。当用1 * 10^5 QS MRSA感染4天的具有3 mm大小伤口的小鼠每隔一天用纯化蔗糖-聚维酮碘治疗时，与MRSA感染和未治疗小鼠的伤口相比，它们的伤口更早闭合并且具有更低的细菌计数。因此，这些结果表明，在糖尿病小鼠皮肤中制备的感染性慢性溃疡是用于监测感染性伤口愈合过程和用于评价药剂功效的良好模型。少

It is clinically well known that bacterial infection delays the healing of chronic skin ulcers. In this study, we succeeded in demonstrating the utility of hereditary diabetic mice as a model for observing the healing process in infectious wounds. infectious chronic cutaneous wounds were prepared by applying MRSA (methicillin-resistant Staphylococcus aureus) to two full-thickness wounds created in the dorsal skin of female G57BLKsJ db/db hereditary diabetic mice. MRSA was applied topically, and the wounds then covered by Cathereep film. Thirteen days after the application, the wounds were histologically evaluated by measuring re-epithelialization (%), area of granulation tissue (mm^2), capillary numbers and bacterial count. Results indicated that the diabetic mice had a high incidence of infectious ulcers and impaired healing compared with normal mice.The following new findings were additionally made : (1) the covered wounds persisted longer and showed more severe manifestations than t … More he open wounds ; (2) a three-mm diameter wound was determined to be the appropriate experimental size for infectious wounds as larger wounds did not close after a one-month incubation period ; (3) 1 *10^6 was determined to be the optimum inoculum size ; (4) an average of four days are required to establish a MRSA infection ; (5) the MRSA-infected wounds remained unhealed after 13 days incubation ; (6) the methicillin-sensitive (MS)SA N315P and MRSA N315PZR strains had a similar pathogenicity with respect to the formation of infectious wounds in diabetic mice. When the mice with 3-mm size wounds, infected with 1 * 10^5 QS MRSA for 4 days, were treated with purified sucrose-povidone iodine every other day, their wounds closed earlier and had a lower bacterial count compared with wounds of the MRSA-infected and non-treated mice. Thus these results indicate that an infectious chronic ulcer prepared in the skin of diabetic mice is a good model for monitoring the healing process of infectious wounds and for evaluating the efficacy of agents. Less