PATHOLOGICAL RESEARCH OF DIABETIC COMPLICATION OF SPONTANEOUS DIABETIC RAT (WBN/Kob)

自发性糖尿病大鼠（WBN/Kob）糖尿病并发症的病理学研究

• 批准号: 09660347
• 负责人: NARAMA Isao
• 金额: $ 0.83万
• 依托单位: SETSUNAN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660347/
• 关键词: DIABETES MELLITUS; DIABETIC COMPLICATION; PATHOLOGY; ANIMAL MODEL

1. Autonomic nerve lesion of WBN/Kob ratAutonomic nerve lesion of spontaneous diabetic WBN/Kob rats is characterized by axonal dystrophy and miniaturization of axon diameter in mesenteric ganglion. These changes are similar to that of human diabetic autonomic neuropathy. Peripheral motor neuropathy of WBN/Kob rat is segmental demyelination. These findings confirmed pathogenesis of peripheral motor neuropathy in WBN/Kob rat is different from that of autonomic neuropathy. However, axon of ileal, colonic, cecal, and vagus nerve increased in size and have no axonal dystrophy. Therefore, it was indicated the influences of diabetes is weak in ileal, colonic, cecal, and vagus nerve.2. Muscle lesion of WBN/Kob ratDespite peripheral nerve abnormalities in WBN/Kob rats, neurogenic abnormality was not seen in muscles.Morphometric analysis of myofiber size by fiber type indicated severe atrophy of type 2a myofibers in extensor digitorum longus muscle, biceps femoris muscle, soleus muscle and diaphragm, hyperirophy of type 2b myofibers in extensor digitorum longus muscle and diaphragm, and hypertrophy of type 1 myofiber in biceps femoris muscle. These changes diffcrcd from age-dependent changes. It may be caused by the diabetic dysmetabolism of anaerobic glycolysis.

1.自发性糖尿病WBN/Kob大鼠的自主神经损害表现为肠系膜神经节轴突营养不良和轴突直径缩小。这些变化与人类糖尿病自主神经病变相似。WBN/Kob大鼠的周围运动神经病变为节段性脱髓鞘病变。这些结果证实了WBN/Kob大鼠周围运动神经病的发病机制不同于自主神经病。而回肠、结肠、盲肠和迷走神经轴突体积增大，无轴突营养不良。说明糖尿病对回肠、结肠、盲肠和迷走神经的影响较弱. WBN/Kob大鼠的肌肉病变尽管WBN/Kob大鼠的周围神经异常，但肌肉中未观察到神经源性异常。按纤维类型进行的肌纤维大小的形态计量学分析表明，趾长伸肌、股二头肌、比目鱼肌和膈肌中的2a型肌纤维严重萎缩，趾长伸肌和膈肌中的2b型肌纤维肥大，和股二头肌中的1型肌纤维肥大。这些变化不同于年龄依赖性变化。它可能是由糖尿病无氧糖酵解代谢障碍引起的。

项目成果

Ozaki Kiyokazu: "Morphologic and hematologic characteristics of storage pool deficiency in beige rat (checliak-Higashi syndrome of rats)" Laboratory Animal Science. 48. 502-506 (1998)

Ozaki Kiyokazu：“米色大鼠储存池缺陷的形态学和血液学特征（大鼠 checliak-Higashi 综合征）”实验动物科学。

Matsuura T: "Morphology and morphometry of the deformed cervical vertebrae in a mutant knotty-tail (knt/knt) mouse" Cong Anom. 38. 67-79 (1998)

Matsuura T：“突变型结尾 (knt/knt) 小鼠变形颈椎的形态学和形态测量” Cong Anom。