Characteristics of angioarchitecture in thyroid tumors in associated with expression of vascular endothelial growth factor (VEGF)

甲状腺肿瘤血管结构特征与血管内皮生长因子（VEGF）表达相关

• 批准号: 09670180
• 负责人: KATOH Ryohei
• 金额: $ 1.28万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670180/
• 关键词: Angioarchitecture; Thyroid; Neoplasms; VEGF; Molecular Pathology; Confocal Laser Scanning Microscopy; 分子病理学; 甲状腺癌; 血管新生

Confocal laser scanning microscopy (CLSM) observation combined with immunohistochemistry for type IV collagen clearly visualized 3-dimensional images of the microvascular structures. This method revealed that the fundamental vascular pattern correlates well with the growth pattern, suggesting an interdependence between parenchyma and stroma characteristic for thyroid tumors. In this respect, we determined to study vascular endothelial growth factor (VEGF) in thyroid tumors. RT-PCR analysis showed that all specimens of thyroid tumors expressed bands corresponding to -121, -165, and 189-amino acid forms of VEGF. Northern blot analysis showed an increase in VEGF mRNA levels in neoplastic tissues in comparison with normal thyroid samples. By nonisotopic in situ hybridization and immunohistochemistry, most of the tumor cells expressed VEGF mRNA and protein, whereas VEGF expression was identified only in epithelium of isolated follicles in normal thyroid tissues. Intense expression of VEGF was observed in overlying tumor cells of neoplastic papillae of papillary carcinoma and in tumor cells surrounding necrosis in undifferentiated carcinoma. Our results suggest that the histological types of thyroid tumor may determine the vascular pattern through a paracrine mechanism involving VEGF.

共聚焦激光扫描显微镜 (CLSM) 观察结合 IV 型胶原的免疫组织化学，清晰地显示了微血管结构的 3 维图像。该方法揭示了基本血管模式与生长模式密切相关，表明甲状腺肿瘤的实质和间质特征之间存在相互依赖性。在这方面，我们决心研究甲状腺肿瘤中的血管内皮生长因子（VEGF）。 RT-PCR分析表明，所有甲状腺肿瘤标本均表达与VEGF-121、-165和189氨基酸形式相对应的条带。 Northern 印迹分析显示，与正常甲状腺样本相比，肿瘤组织中 VEGF mRNA 水平有所增加。通过非同位素原位杂交和免疫组化，大多数肿瘤细胞表达VEGF mRNA和蛋白，而仅在正常甲状腺组织中分离滤泡的上皮细胞中发现VEGF表达。在乳头状癌的肿瘤性乳头的上覆肿瘤细胞和未分化癌的坏死周围的肿瘤细胞中观察到VEGF的强烈表达。我们的结果表明，甲状腺肿瘤的组织学类型可能通过涉及 VEGF 的旁分泌机制决定血管模式。

项目成果

Ryohei Katoh, Eri Miyagi, et al.: "Expression of vascular endothelial growth factor (VEGF) in thyroid neoplasms."Human Pathology. 30. 891-897 (1999)

Ryohei Katoh、Eri Miyagi 等人：“甲状腺肿瘤中血管内皮生长因子 (VEGF) 的表达。”人类病理学。

Ryohei Katoh, et al: "Expression of vascular sndothelial growth factor (VEGF) in thyroid neoplasms"Human pathology. 30. 891-897 (1999)

Ryohei Katoh 等人：“甲状腺肿瘤中血管内皮生长因子 (VEGF) 的表达”人类病理学。