Epstein-Barr virus latent membrane protein-1 oncogene deletions in Hodgkin's disease and gastric carcinoma in Japan and Nepal.

日本和尼泊尔霍奇金病和胃癌中 Epstein-Barr 病毒潜伏膜蛋白 1 癌基因缺失。

• 批准号: 09670177
• 负责人: ISHII Genichiro
• 金额: $ 1.86万
• 依托单位: SCHOOL OF MEDICINE, CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670177/
• 关键词: Epstein-Barr virus; Latent membrane protein-1; Hodgkin's disease; Gastric carcinoma; EBウイルス; LMP-1; ホジキン病; 胃癌; 欠失LMP-1遺伝子; EBV種地域差; EBV関連胃癌; bcl-2免疫染色

The presence of 30-bp deletions within the carboxy terminal end of the LMP-1 oncogene of EBV has been reported in EBV-aasociated neolasms. We analysed the 30-bp deletion of the LMP-1 gene in Hodgkin's disease and gastric carcinoma in Japan and Nepal. 35% of Hodgkin's cells in Japan showed positive reaction for LMP-1. Direct sequence analysis of the PCR products of carboxy terminal end of the LMP-1 oncogene revealed identical 30-bp deletion in 5 of 6 samples of EBV-associated Hodgkin's disease. In EBV-associated gastric carcinoma in Japan, this deletion was found in 2 of 3 samples.On the other hand, 100% of Hodgkin's cells showed positive reaction for LMP-1 in Nepal. Identical 30-bp deletion was observed in 4 of 8 samples.Furthermore we examined the proliferating activity and P53 expression of Hodgkin's cells with and without 30-bp deletion. However proliferating activity and P53 expression was almost similar between these two groups.Our results suggest that the 30-bp deletion mutant is widely spread in Japan and Nepal and is not implicated in EBV-associated neolasms.

在EB病毒相关的新瘤中，已经报道了EB病毒LMP-1癌基因羧基末端内30-bp缺失的存在。我们分析了日本和尼泊尔霍奇金病和胃癌中LMP-1基因的30 bp缺失。日本35%的霍奇金淋巴瘤细胞显示LMP-1阳性反应。6例EBV相关性霍奇金病中5例LMP-1癌基因羧基端缺失。在日本的EBV相关胃癌中，3例标本中有2例发现这种缺失，而在尼泊尔的霍奇金淋巴瘤细胞中，100%的LMP-1呈阳性反应。在8例标本中有4例发现30-bp缺失，并进一步检测了30-bp缺失前后霍奇金淋巴瘤细胞的增殖活性和P53蛋白表达。我们的研究结果表明30-bp缺失突变体在日本和尼泊尔广泛分布，并且与EBV相关的neolasms无关。

项目成果

Ishii G: "Interbukin-II gene expression in human lpmphoid mabgnanuy" Hematology. 3. 143-151 (1998)

Ishii G：“人淋巴组织中的 Interbukin-II 基因表达”血液学。

Isobe, K: "Clinicopathological evaluation of the Revired European-American classification of lymphid Neoplams(REAL) in Japan" Lenkemia and lymphoma. in press

Isobe, K：“日本修订的欧美淋巴肿瘤分类 (REAL) 的临床病理学评估” 白血病和淋巴瘤。