大腸発がんにおける食事・腸内細菌叢・代謝物の複合的作用の解明を目指した疫学研究

流行病学研究旨在阐明饮食、肠道菌群和代谢物对结直肠癌发生的综合影响

• 批准号: 22K19680
• 负责人: 岩崎 基
• 金额: $ 4.08万
• 依托单位: National Cancer Center Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Research (Exploratory)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-06-30 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19680/
• 关键词: 大腸; 腸内細菌叢; 代謝物; 食事因子; 疫学研究; 大腸腫瘍

本研究では、無症状の住民を対象に大腸内視鏡検診を実施した大島研究において、検診前に収集した生活習慣・食事に関するアンケート調査結果、便および血漿検体の分析結果を用いて、食事因子・腸内細菌・代謝物と大腸腫瘍リスクとの関連を複合的作用の観点から検討する事を目的としている。今年度は、一次・二次・三次胆汁酸の濃度（抱合型および遊離型）の測定を実施する対象者の選定を実施した。大腸腫瘍症例125人（がん30例、高度異形腺腫26例、10mm以上の腺腫69例）とランダムサンプリングした対照群194人の計319人のうち、血漿検体が利用可能な303人を対象とした。また血漿測定は、本研究で対象とする胆汁酸を含む約500の代謝物を対象に液体クロマトグラフ質量分析計による測定を開始した。また、これらの対象者について、66食品から構成される食物摂取頻度調査票の回答結果を用いて肉類・脂肪酸の摂取量等を算出し、生活習慣および食事関連のデータの整備を実施した。さらに、先行する別研究で実施した便試料由来DNAを用いた16S rRNA解析のデータの整備も実施し、解析用のデータセットの構築を行っている。これらのデータを用いて、①肉類・脂肪酸摂取量、②腸内細菌叢の情報、③血漿中胆汁酸濃度のそれぞれについて大腸腫瘍リスクとの関連を検討する。さらに①と大腸腫瘍リスクとの関連において②を介する場合、②と③を介する場合の関連の検討を行い、食事因子・腸内細菌・代謝物と大腸腫瘍リスクとの関連を複合的作用の観点から解析する予定である。

This study aims to investigate the effects of food factors, intestinal bacteria, metabolites, and colorectal cancer on colorectal endoscopy in asymptomatic residents, as well as on the effects of dietary habits, dietary habits, and plasma samples before diagnosis in the Big Island Study. This year, the concentration of primary, secondary and tertiary bile acids (inclusive and free) was determined and the target was selected. There were 125 cases of colorectal tumors (30 cases of colon tumors, 26 cases of high-grade atypical adenomas, and 69 cases of adenomas larger than 10 mm) and 319 of the 194 people in the control group, including 303 people whose plasma samples could be used. The determination of bile acids in plasma was initiated by mass spectrometry with a concentration of approximately 500. 66 Food composition, food frequency survey results, food habits, food related preparation, etc. In this study, we conducted a pilot study on the preparation and construction of samples derived from DNA analysis using 16S rRNA. The relationship between the concentrations of bile acids in plasma and the concentrations of fatty acids in colon cancer was discussed. (1) The relationship between colon cancer and intestinal bacteria;(2) The relationship between colon cancer and intestinal bacteria;(3) The relationship between colon cancer and intestinal bacteria;(4) The relationship between intestinal bacteria and intestinal bacteria;(5) The relationship between intestinal bacteria and intestinal bacteria;(6) The relationship between intestinal bacteria and intestinal bacteria;(7) The relationship between intestinal bacteria and intestinal bacteria;(8) The relationship between intestinal bacteria and intestinal bacteria;(9) The relationship between intestinal bacteria and intestinal bacteria;(9) The relationship between intestinal bacteria and intestinal bacteria;(10) The relationship between intestinal bacteria;(11) The relationship between intestinal bacteria and intestinal cancer;(12) The relationship between intestinal bacteria and intestinal bacteria;(13) The relationship between intestinal bacteria;(11) The relationship between intestinal bacteria and intestinal bacteria;(12) The relationship between intestinal bacteria;(11) The relationship between intestinal bacteria;(12) The relationship between intestinal bacteria;(13) The relationship between intestinal bacteria;(11) The relationship between intestinal bacteria;(12) The relationship between intestinal bacteria;(13) The relationship between intestinal bacteria;(

项目成果

Plasma biomarker studies on cancer within the Japan Public Health Center-based prospective study.

基于日本公共卫生中心的前瞻性研究中关于癌症的血浆生物标志物研究。

• 发表时间: 2022
• 作者: Fujimoto Shuntaro;Hamano Yuya;Ichioka Kentaro;Manabe Sho;Hirai Eriko;Ogawa Osamu;Tamaki Keiji;Motoki Iwasaki
• 通讯作者: Motoki Iwasaki