Studies on DNA intercalators using modified cyclodextrins

改性环糊精DNA嵌入剂的研究

• 批准号: 09650869
• 负责人: IKEDA Tsukasa
• 金额: $ 2.11万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650869/
• 关键词: cyclodextrin; modified cyclodextrin; anthoracene modified cyclodextrin; DNA intercalator; complex

Cyclodextrins (CDs) are cyclic oligosaccharides consisting of at least six glucopyranose units. CDs have been extensively studied as host molecules for molecular recognition and encapsulations of various compounds, especially drugs and drug delivery systems, because CD has a hydrophobic cavity in which CD can bind various guest molecules. For development of molecular recognition using modified CDs with specific and/or selective affinities for biomolecules and also for the interest of DNA intercalation and drug delivery systems, four kinds of anthryl (alkylamino)-β-CDs were synthesized. Guest-induced conformational changes of them were observed by measurements of ^1H-NMR spectra and circular dichroism spectra. Their abilities of binding to DNA were also observed by measurements of ^1H-NMR spectra, UV spectra and fluorescence spectra. Such modified β-CDs themselves do not bind to DNA, but, after making a complex with a guest molecule, such complexes show abilities of binding to DNA.It shows a new principle of an chemically switched DNA binding system.

环糊精（CD）是由至少六个吡喃葡萄糖单元组成的环状低聚糖。CD作为主体分子被广泛研究，用于各种化合物，特别是药物和药物递送系统的分子识别和修饰，因为CD具有疏水空腔，其中CD可以结合各种客体分子。为了开发对生物分子具有特异性和/或选择性亲和性的修饰环糊精的分子识别功能，以及用于DNA嵌入和药物传递系统，合成了4种蒽基（烷氨基）-β-环糊精。通过核磁共振氢谱和圆二色谱的测定，观察到客体诱导的构象变化。通过~ 1H-NMR谱、紫外光谱和荧光光谱的测定，观察了它们与DNA的结合能力。这种修饰的β-CD本身不与DNA结合，但与客体分子形成复合物后，这种复合物显示出与DNA结合的能力，这表明了化学开关DNA结合系统的新原理。

项目成果

K.Hamasaki: "Dansyl-Modified Cyclodextrins as Fluorescent Chemosensors for Molecular Recognition"Supramol.Chem.. Vol.8. 125-135 (1997)

K.Hamasaki：“丹酰基修饰环糊精作为分子识别的荧光化学传感器”Supramol.Chem.. 第 8 卷。

S.Usui, K.Hamasaki, T.Kuwabara, A.Nakamura: "Molecular Dynamics Conformational Search for the Factors that Determine Conformation of Modified Cyclodextrins"Supramol.Chem.. Vol.9. 57-67 (1998)

S.Usui、K.Hamasaki、T.Kuwabara、A.Nakamura：“分子动力学构象搜索决定修饰环糊精构象的因素”Supramol.Chem.. Vol.9。

A.Kuroda: "Inorganic polyphosphate kinase is required to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli"Proceedings of the National Academy of Sciences of USA. 96. 14264-14269 (1999)

A.Kuroda：“无机多磷酸激酶是刺激蛋白质降解和适应大肠杆菌氨基酸饥饿所必需的”美国国家科学院院刊。

K.Hamasaki: "Dansyl-Modified Cyclodextrins as Fluorescent Chemosensors for Molecular Reagrition" Supramol.Chem.8. 125-135 (1997)

K.Hamasaki：“丹酰基修饰的环糊精作为分子重聚的荧光化学传感器”Supramol.Chem.8。

T.Aoyagi: "Alizarin Yellow Modified β-Cyclodextrin as a Guest Responsive Absorption-Change Sensor"Anal.Chem.. Vol.69. 659-663 (1997)

T.Aoyagi：“茜素黄改性 β-环糊精作为客体响应吸收变化传感器”Anal.Chem.. 第 69 卷 659-663 (1997)。