Synthesis and Performance Analysis of Inhibitors for Quorum Sensing in Bacteria

细菌群体感应抑制剂的合成及性能分析

• 批准号: 14550772
• 负责人: IKEDA Tsukasa
• 金额: $ 2.56万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550772/
• 关键词: Bacteria; Quorum Sensing; Autoinducer; N-acyl-L-homoserinnlactone; Inhibitor; Cell-cell communication; Regulation of gene expression; Gram-negative bacteria; N-アシル-L-ホモセリンラクトン; 細胞間情報伝達機構; 緑膿菌

Quorum sensing is one of the cell-cell communication mechanisms depending on cell population density in gram-negative bacteria. Several kinds of N-acyl-L-homoserine lactones (AHLs) have been identified as signal compounds involved in this mechanism and called autoinducers. AHLs are produced in bacteria, diffused outside and inside of bacteria and regulate expression of genes responsible for bioluminescence, secretion of virulence factors, expression of pathogenicities and forming biofilm, respectively. Molecular genetics of quorum sensing have been extensively studied, but there exists little information about the interaction of AHLs with transcriptional activator proteins with respect to their structures and responses.To develop the control methods of quorum sensing mechanisms, several kinds of synthetic AHL analogues have been reported but showed low activities as antagonists of quorum sensing. Acylated cyclopentyl amide(Cn-CPA) were synthesized as Ahl analogues. C8-CPA acted as an antagonist of quorum sensing in Pseudomonas aeruginosa las and rhl system and also showed inhibitory effects on biofilm formation by P.aeruginosa.

在革兰氏阴性菌中，群体感应是依赖于细胞群体密度的细胞间通讯机制之一。几种N-酰基-L-高丝氨酸内酯(AHL)已被鉴定为参与这一机制的信号化合物，称为自身诱导剂。AHL在细菌中产生，扩散到细菌内外，分别调节与生物发光、毒力因子分泌、致病性表达和生物膜形成有关的基因的表达。群体感应的分子遗传学已经得到了广泛的研究，但关于AHL与转录激活蛋白的相互作用在结构和反应方面的信息很少。为了建立群体感应机制的控制方法，报道了几种合成的AHL类似物，但它们作为群体感应的拮抗剂活性较低。合成了酰化环戊酰胺(CN-CPA)作为AHL类似物。C8-CPA在铜绿假单胞菌LAS和rhL系统中起到群体感应拮抗剂的作用，对铜绿假单胞菌生物被膜的形成也有抑制作用。

项目成果

ナノマテリアル・シクロデキストリン 第IV編-1「シクロデキストリンによる微生物の細胞間情報伝達機構制御」

纳米材料环糊精第IV-1部分“环糊精控制微生物细胞间通讯机制”

• 发表时间: 2005
• 作者: 池田宰;加藤紀弘(分担)
• 通讯作者: 加藤紀弘(分担)

Uptake of signal molecules for gram-negative bacterial cell-to-cell communication on polymer gels

在聚合物凝胶上摄取革兰氏阴性细菌细胞间通讯的信号分子

• 发表时间: 2005
• 期刊: Transactions of the Materials Research Society of Japan 30・3
• 作者: 米田博幸;淺野和典;Norihiro Kato;Norihiro Kato;Norihiro Kato;Norihiro Kato;Norihiro Kato
• 通讯作者: Norihiro Kato

Quorum sensing control using synthetic autoinducer analogues and cyclodextrins in Gram-negative bacteria

在革兰氏阴性细菌中使用合成自诱导剂类似物和环糊精进行群体感应控制

• 发表时间: 2004
• 期刊: Proceedings of APPChE 2004 3P-01-053
• 作者: T.Ikeda;T.Morohoshi;N.Kato;M.Inoyama;S.Nakazawa;K.Hiratani;T.Ishida;J.Kato;H.Ohtake
• 通讯作者: H.Ohtake

Regulation of quorum sensing in Serratia marcescens by adsorption of N-acylhomoserine lactones on cyclodextrin immobilized gel

环糊精固定化凝胶吸附 N-酰基高丝氨酸内酯调节粘质沙雷氏菌群体感应

• 发表时间: 2005
• 期刊: Transactions of the Materials Research Society of Japan 30・3
• 作者: 米田博幸;淺野和典;Norihiro Kato;Norihiro Kato
• 通讯作者: Norihiro Kato

N.Takiguchi, T.Tajima, K.Asayama, T.Ikeda, A.Kuroda, J.Kato, H.Ohtake: "Behavioral Responses of the Ciliated Protozoan Paramecium caudatum to 2,4-Dichlorophenoxyacetic Acid and Its Analogues"J. Biosci. Bioeng.. 93・4. 416-420 (2002)

N.Takiguchi、T.Tajima、K.Asayama、T.Ikeda、A.Kuroda、J.Kato、H.Ohtake：“纤毛原生动物草履虫尾部对 2,4-二氯苯氧乙酸及其类似物的行为反应”J。生物科学.. 93・420 (2002)