Receptor-functionalized Micelles and Vesicles of Amphiphilic Perylene Bisimide Dyes

通过两亲性的苝酰双亚胺（PBI）的共自组装制备水溶性胶束和囊泡。水溶性PBI自组装体将在内部负载pH敏感的荧光供体，并在表面用冠醚受体或带正电荷的精胺基团官能化。这些功能组装体将通过PBI结构单元侧链上双键的原位光聚合而稳定，并得到一种新型的带有多价识别位点的胶束纳米颗粒。基于荧光共振能量转移或光诱导电子转移机制，这些水溶性功能胶束和囊泡将显示出对激发光颜色和强度的响应性变化，从而可以发展成为对pH（氢离子）、碱金属阳离子或生物大分子（如DNA）有效的、比率式的和选择性的荧光传感器。通过使用荧光共聚焦显微镜，这种功能性荧光传感器将提供氢离子（质子）、阳离子和生物大分子DNA的位置、相互作用、运输、分布和动力学的直接可视化。

Water-soluble micelles and vesicles will be prepared by co-self-assembly of amphiphlic perylene bisimides (PBI). The water-soluble PBI self-assemblies will be loaded with pH-sensitive fluorescence donors in the interior, and functionalized with crown ether receptors or positively charged spermine groups at the surface. These functional assemblies will be stabilized by in-situ photopolymerization of the double bonds at the side chain of PBI building block, and to give a new type of micellar nanoparticles bearing multivalent recognition sites. On the basis of fluorescence resonance energy transfer or photo-induced electron transfer mechanism, these water-soluble functional micelles and vesicles will display stimuliresponsive changes in emission color and intensity, and thus can be developed as effective, ratiometric and selective fluorescence sensors towards pH (hydrogen ion), alkali metal cation, or biomacromolecules such as DNA. By using fluorescence confocal microscopy, such functional fluorescence sensors will provide a direct visualization of the location, interaction, transport, distribution and dynamics of hydrogen ion (proton), cation, and biomacromolecule DNA.