Phylogenetic and evolutionary study of the Erysiphaceae based on rDNA analyses

基于 rDNA 分析的白粉菌科系统发育和进化研究

• 批准号: 09660048
• 负责人: TAKAMATSU Susumu
• 金额: $ 2.43万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660048/
• 关键词: powdery mildew; phylogeny; evolution; nucleotide sequence; rDNA; ITS; 植物; 病原菌; 寄生

The nucleotide sequences of the nuclear ribosomal DNA including 18S, 5.8S, and 28S rDNA and the internal transcribed spacer regions were determined for 33 powdery mildew taxa spanning 15 genera to infer the phylogenetic relationships for these fungi. Phylogenetic analyses revealed that Uncinula septata was placed in the primitive base to the large clade composed of all other powdery mildew taxa. Powdery mildews excluding Unc. septata were split into five major lineages. The Pseudoidium lineage consisted of six genera, i.e., Erysiphe, Microsphaera, Uncinula, Unciunliella, Brasiliomyces, and Typhulochaeta, all of which are characterized by polyascal cleistothecia and Pseudoidium-type anamorph. The Eudoidium lineage consisted of three Erysiphe species (E.cichoracearun, E.orontii and E.galeopsidis) and Arthrocladiella mougeotii, which are characterized by polyascal cleistothecia and Euoidium-type anamorph. The endophytic lineage consisted of two Phyllactinia species (Phy.moricola and Phy.k … More akicola), Leveillula taurica and Pleochaeta shiraiana, which are characterized by the presence of endophytic or partly endophytic mycelia. The fibrosin lineage consisted of the genera Sawadaea, Cystotheca, Podosphaera, and Sphaerotheca, all of which are characterized by the presence of well-developed fibrosin bodies in the conidia and conidiophores. The monocot lineage consisted of a single species, Blumeria graminis, which is well characterized by being parasitic to monocots, especially to cereal plants. Character polarization was discussed on several major characteristics. The mycelioid appendage which has long been regarded as an ancestral character may be a derived character as a result of an event that may have occurred multiple times independently due to convergence. Euoidium-type anamorph and clavatc appressorial germ tube are considered to be ancestral characters to Pseudoidium-type anamorph and lobed germ tube. Ectophytic nature and polyascal cleistothecia are also regarded as ancestral features to endophytic nature and monoascal cleistothecia. Less

测定了15个属33个白粉病分类群的18S、5.8S和28S rDNA及内转录间隔区的核苷酸序列，以推断这些真菌的系统发育关系。系统发育分析表明，钩丝壳septata被放置在原始基地的大分支组成的所有其他白粉病类群。白粉病，不包括Unc。septata被分成五个主要的谱系。Pseudoidium谱系由六个属组成，即，白粉菌属（Erysiphe）、微球菌属（Microsphaera）、钩丝壳属（Uncinula）、钩丝壳属（Unciunliella）、巴西酵母属（Brasiliomyces）和盲囊壳属（Typhulochaeta），它们都具有多囊闭囊壳和假囊壳型无性型的特征。Eudoidium谱系由三个白粉菌属种（E.cichoracearun，E.orontii和E.galeopsidis）和Arthrocladiella mougeotii组成，其特征是多囊闭囊藻和Euoidium型无性型。结果表明，该菌的内生谱系由两个种（Phy.moricola和Phy.k ...更多信息 akicola）、Leveillula taurica和Pleochaeta shiraiana，其特征在于存在内生或部分内生菌丝体。纤维蛋白酶谱系由Sawadaea、Cystotheca、Podosphaera和Sphaerotheca属组成，所有这些属的特征在于分生孢子和分生孢子梗中存在发育良好的纤维蛋白酶体。单子叶植物谱系由一个物种组成，即禾本科布氏菌（Blumeria graminis），其特征在于寄生于单子叶植物，特别是谷类植物。讨论了几个主要特征的特征极化。菌丝状附属物长期以来一直被认为是一个祖先的字符可能是一个衍生的字符作为一个事件的结果，可能已经发生了多次独立的收敛。Euoidium型无性型和棒状附着胞芽管被认为是Pseudoidium型无性型和裂芽芽管的祖先特征。外生性和多囊闭囊藻也被认为是内生性和单囊闭囊藻的祖先特征。少

项目成果

Mori,Y.: "Evolutionary analysis of the powdery mildew fungi(Erysiphales)using nucleotide sequences of the nuclear ribosomal DNA."Mydologia. 92. 74-93 (2000)

Mori, Y.：“利用核核糖体 DNA 的核苷酸序列对白粉病真菌（白粉菌）进行进化分析。”Mydologia。

Khodaparast,S.A.: "Phylogenetic structure of the genus Leveillula (Erysiphales : Erysiphaceae) inferred from the nucleotide sequences of the rDNA ITS region with special references to the Leveillula taurica species complex."Mycal.Res.. 105(in press). (200

Khodaparast，S.A.：“根据 rDNA ITS 区域的核苷酸序列推断出白粉菌属（白粉菌目：白粉菌科）的系统发育结构，特别提及鞑靼白粉菌物种复合体。”Mycal.Res.. 105（出版中）。

Takamatsu, S.: "Phylogenetic analysis and predicted secondary structures of the rDNA internal transcribed spacers of the powdery mildew fungi (Erysiphaceae)."Mycoscience. 39. 441-453 (1998)

Takamatsu, S.：“白粉病真菌（白粉菌科）的 rDNA 内转录间隔区的系统发育分析和预测的二级结构。”Mycoscience。

Cunnington,J.H.: "Molecular identification of anamorphic powdery mildews (Erysiphales)."Mycol.Res.. 105(in press). (2001)

Cunnington, J.H.：“变形白粉病（白粉菌）的分子鉴定。”Mycol.Res.. 105（印刷中）。

Takamatsu,S.: "PCR primers useful for nucleotide sequencing of rDNA of the powderymildew fungi."Mycoscience. 42(in press). (2001)

Takamatsu,S.：“PCR 引物可用于白粉病真菌 rDNA 的核苷酸测序。”Mycoscience。