Studies on Anti-disease Factors Produced by Fish Intestinal Bacteria

鱼肠道细菌产生抗病因子的研究

• 批准号: 09660214
• 负责人: SUGITA Haruo
• 金额: $ 2.05万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660214/
• 关键词: Intestinal bacteria; Anti-disease factor; Antibacterial activity; Marine fish; Freshwater fish; Protease; Acetic acid; Neuraminidase; Vibrio属細菌; 胆汁酸抵抗性; 低pH抵抗性; 魚類; ヒラメ; 魚類病原細菌; Aeromonas属細菌; タンパク性抗菌物質; ジデロフォア; 魚病細菌; Bacillus属細菌

Anti-disease factors produced by fish intestinal bacteria were investigated and the following results were obtained:1. Most Vibrio bacteria in Japanese flounder-rearing seawater were suggested to derive from fish intestines via fecal pellets.2. Clostridia and gram-positive anaerobic cocci isolated from coastal fish intestines seem to adapt to the environment inside intestinal tracts of fish.3. Of bacteria derived from fish intestines, water and sediments, 1.0-15% of isolates showed antibacterial activities against fish pathogenic bacteria.4. Antibacterial substances produced by Vibrio sp. NM 10 and Bacillus sp. NM 14 were a proteinous substance and a sideerophore, respectively.5. As many of 88-94% of Aeromonas isolates produced acetic acid, which may be an antibacterial subtance.6. Many isolates of genera Aeromonas and Vibrio produced at least two types of protease, serine protease and metalloprotease.7. About 10% of vibrios isolates from larvae and juveniles of Japanese flounder produced neuraminidase.

对鱼肠道细菌产生的抗病因子进行了调查，得到了以下结果：1.养殖比目鱼海水中的弧菌大多是通过粪便颗粒从鱼肠道中产生的。从近海鱼类肠道分离的梭状芽胞杆菌和革兰氏阳性厌氧菌似乎适应鱼类肠道内的环境。在来自鱼肠、水和底泥的细菌中，1.0-15%的分离物对鱼类病原菌具有抑菌活性。弧菌产生的抗菌物质。NM 10和芽孢杆菌属(Bacillussp.)NM14分别是一种蛋白物质和一种侧体。88-94%的气单胞菌产生醋酸，这可能是一种抗菌物质。气单胞菌属和弧菌属的许多分离株至少产生两种类型的蛋白酶：丝氨酸蛋白酶和金属蛋白酶。从牙鲆仔稚鱼分离的弧菌中，约有10%的菌株产生神经氨酸酶。

项目成果

H. Sugita: "Introduction to Environmental Science"Kodansha. 212 (1997)

H.杉田：《环境科学概论》讲谈社。

H. Sugita: "Production of amylase by the intestinal microflora in cultured freshwater fish."Lett, Appl. Microbiol.. 24. 105-108 (1997)

H. Sugita：“养殖淡水鱼肠道微生物群产生淀粉酶。”Lett，Appl。

Y. Konagaya: "Preliminary investigation of β-N-acetylglucosaminidase from a marine bacterium, Clostridium sp. Strain F-8."Suisanzoshoku. 46. 593-594 (1998)

Y. Konagaya：“来自海洋细菌梭状芽胞杆菌菌株 F-8 的 β-N-乙酰氨基葡萄糖苷酶的初步研究”Suisanzoshoku，46. 593-594 (1998)

H.Sugita: "Vibrio sp. Strain NM10, isolated from the intestines of Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida"Appl. Environ. Microbiol. 63. 4986-4989 (1997)

H.Sugita：“从日本沿海鱼类肠道中分离出的弧菌菌株 NM10，对杀鱼巴斯德氏菌具有抑制作用”。

杉田治男: "循環ろ過式水槽水の細菌相に及ぼすヒラメ稚魚の影響"水産増殖. 46. 237-241 (1998)

Haruo Sugita：“幼鱼对循环过滤水族箱水中细菌菌群的影响”水产养殖。 46. 237-241 (1998)