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A study of development of probiotics for fish

鱼类益生菌的开发研究

基本信息

批准号：
16580160
负责人：
SUGITA Haruo
金额：
$ 2.37万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580160/
关键词：
probiotics fish intestinal bacteria opportunistic bacteria antibacterial activity self-defense mechanisms Jananese flounder rainbow trout 腸内細菌叢 Listonella anguillarum Vibrio属細菌 Aeromonas属細菌ワムシ Lactobacillus rhamnosus 分類

项目摘要

1.Ecology of a fish pathogen Listonella anguillarum in a seed production facility for the Japanese flounder was investigated for the better understanding of behavior of opportunistic pathogens in aquaculture environments. L.anguillarum associated with larvae and juvenile seems to be derived from live diets including rotifers and rearing waters. This result idicates the importance of probiotic practice for the prevention of opportunistic infections caused by bacteria such as L.anguillarum.2.The culturability of intestinal bacteria from eight coastal fish as determined by both the culture method and the direct count method using DAPI ranged from 0.00003 to 80.9%. Predominant intestinal bacteria in the mullet as determined by the FISH method were Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria and Gram-positive bacteria. Taken together, it is found that the intestinal microflora of coastal fish is composed of different phyla of bacteria.3.Intestinal tracts of Japanese flounder were mainly colonized by Vibrio scophthalmi-V.ichthyoenteri group and V.fischeri, amost all of which digested the colloidal chitin. On the other hand, major intestinal bacteria in the production of antibacterial substances were V.harveyi, V.ichthyoenteri and V.fischeri.4.In the rainbow trout fed with live cells of probiotic Lactobacillus rhamnosus JCM 1136, the serum lysozyme and complement activity, and the phagocytic activity of head kidney leucocytes were significantly enhanced compared with control fish. These results strongly suggest that L.rhamnosus possesses the ability to immunomodulate the fish.5.These results obtained through the above experiments show that probiotic treatment is the important method to prevent the opportunistic infections in fish and aquatic animals.

1.为了更好地了解条件致病菌在水产养殖环境中的行为，对一种鱼类病原菌--鳗鱼李斯特氏菌(Listonella Angillarum)在养殖设施中的生态进行了调查。与幼虫和幼鱼有关的鳗弧菌似乎来自包括轮虫和饲养水域在内的活饲料。这一结果表明了益生菌实践对于预防由鳗鱼乳杆菌等细菌引起的机会性感染的重要性。2.用培养法和直接计数法测定8种近海鱼类肠道细菌的培养能力在0.00003%到80.9%之间。用FISH方法测定的乌鱼肠道细菌主要是α-蛋白杆菌、β-蛋白细菌、γ-蛋白细菌、三角洲蛋白细菌和革兰氏阳性细菌。综上所述，近海鱼类肠道微生物区系由不同的细菌门组成。3.牙鲆肠道主要由弧菌群和鱼肠弧菌定植，均主要消化胶体甲壳素。另一方面，产生抗菌物质的主要肠道细菌是哈维氏弧菌、鱼肠弧菌和裂隙弧菌。4.在投喂鼠李糖乳杆菌JCM 1136活细胞的虹鳟鱼中，血清溶菌酶和补体活性以及头肾白细胞的吞噬活性均显著高于对照鱼。这些结果有力地表明，鼠李糖具有免疫调节鱼类的能力。5.上述实验结果表明，益生菌处理是预防鱼类和水生动物机会性感染的重要方法。

项目成果

期刊论文数量（51）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Occurrence of Listonella anguillarum in seed production environments of Japanese flounder Paralichthys olivaceus (Temminek et Schlegel)

鳗鱼利斯顿氏菌在牙鲽牙鲆 (Temminek et Schlegel) 种子生产环境中的出现

DOI：
发表时间：
2005
期刊：
Aquaculture Research 36
影响因子：
0
作者：
Leong;S.C.Y.;S.Taguchi.;H.Sugita et al.
通讯作者：
H.Sugita et al.

Occurrence of Lstonella anguillarum in seed production environments of Japanese flounder Paralichthys olivaceus (Temminck et Schlegel)

日本牙鲆Paralichthys olivaceus (Temminck et Schlegel) 种子生产环境中存在鳗鱼杆菌 (Temminck et Schlegel)

DOI：
发表时间：
2005
期刊：
Aquaculture Research 36・9
影响因子：
0
作者：
Fukui;A;Tsuchiya;T;Kuroda;H;Uotani;I;A.Shiinaほか3名;A.Panigrahiほか5名;H.Sugitaほか3名
通讯作者：
H.Sugitaほか3名

海の環境微生物学

海洋环境微生物学