Molecular taxonomy of intestinal bacteria from freshwater fish and its application

淡水鱼肠道细菌的分子分类及其应用

• 批准号: 12660180
• 负责人: SUGITA Haruo
• 金额: $ 2.3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660180/
• 关键词: fish; intestinal bacteria; 16S rRNA; FISH method; Phylogeny; culture technique; freshwater fish; antibacterial activity; 粘液付着性; ティラピア; ソウギョ; 16SrRNA; キンギョ

Intestinal bacteria of fish was investigated and the following results were obtained.1. Phylogeny of intestinal bacteria isolated from goldfish were examined based on 16S rDNA sequences and they were divided into eight clusters : Aeromonas hydrophiila, Aeromonas sp., Citrobacter freundii, Shewanella sp., Vibrio sp., Vagococcus sp. and Fusobacteria and CFB group.2. Similarly, intestinal bacteria from tilapia and grass carp were mainly classified into five clusters : Aeromonas veronii, Citrobacter freundii, Plesiomonas shigelloides, Fusobacteria and CFB group.3. Cotrary to this, intestinal tract from Japanese flounder and red seabream were mainly divided into three clusters : Vibrio fischeri, V. scophthalmi and V. carchiae.4. Bacterial populations in goldfish feces were analyzed with fluorescentl in situ hybridization method and it was found that goldfish harbors α-, β- and γ-Proteobacteria at high densities. Probes AER66 and BAC303 could detect Aeromonas and Bacteroides type A, respectively.5. Antibacterial abilities of intestinal bacteria from Japanese flounder were determined using 4 fish pathogens, and it was found that 1.7-24.3% of intestinal bacteria possess the activities.

对鱼类肠道细菌进行了调查，得到以下结果.根据16 S rDNA序列分析，金鱼肠道细菌可分为8个类群：嗜水气单胞菌（Aeromonashydrophila）、气单胞菌属（Aeromonassp.）弗氏柠檬酸杆菌，希瓦氏菌属，弧菌属，迷走球菌属（Vagococcus sp.）和梭杆菌属（Fusobacteria）和CFB类群。罗非鱼和草鱼肠道细菌主要分为5个类群：Veronii气单胞菌群、弗氏柠檬酸杆菌群、类志贺邻单胞菌群、梭杆菌群和CFB群.与此相反，牙鲆和红石斑鱼肠道主要分为三个菌群：费氏弧菌、大眼弧菌和卡氏弧菌.应用荧光原位杂交技术对金鱼粪便中的细菌种群进行了分析，发现金鱼粪便中含有高密度的α-、β-和γ-变形菌。AER 66和BAC 303探针可分别检测气单胞菌和A型拟杆菌.用4种鱼类病原菌测定了比目鱼肠道细菌的抗菌能力，发现1.7-24.3%的肠道细菌具有抗菌活性。

项目成果

H. Sugita, Y. Shinagawa, and R. Okano: "Neuraminidase-producing ability of intestinal bacteria isolated from coastal fish"Lett. Appl. Microbiol.. 31. 10-13 (2000)

H. Sugita、Y. Shinakawa 和 R. Okano：“从沿海鱼类中分离出的肠道细菌产生神经氨酸酶的能力”Lett。

C.Tsuchiya, T.Sagesaka, Y.Ito, T.Yamaguchi, H.Sugita: "Identification of goldfish intestinal bacteria 16S ribosomal DNA sequence analysis"水産増殖. 50(印刷中). (2002)

C.Tsuchiya、T.Sagesaka、Y.Ito、T.Yamaguchi、H.Sugita：“金鱼肠道细菌 16S 核糖体 DNA 序列分析的鉴定”水产养殖 50（印刷中）。

H. Sugita, M. Sakamoto, Y. Shirasu, A. Takizawa, N. Mano and R. Nakashima: "Neuraminidase-producing bacteria from the intestinal tract of coastal fishes"Suisanzoshoku. 49(4). 511-512 (2001)

H. Sugita、M. Sakamoto、Y. Shirasu、A. Takizawa、N. Mano 和 R. Nakashima：“沿海鱼类肠道中产生神经氨酸酶的细菌”Suisanzoshoku。

M.Asfie,T.Ishigaki,R.Okano,N.Akiyama,and H.Sugita: "Antibacterial abilities of microflora in the Japanese flounder-rearing aquaria"水産増殖. 48・2. 227-231 (2000)

M. Asfie、T. Ishigaki、R. Okano、N. Akiyama 和 H. Sugita：“日本比目鱼养殖水族箱中微生物群的抗菌能力” 48・2（2000 年）。

M. Asfie, T. Ishigaki, R. Okano, N. Akiyama and H. Sugita: "Antibacterial abilities of microflora in the Japanese flounder-rearing aquaria"Suisanzoshoku. 48(2). 227-231 (2000)

M. Asfie、T. Ishigaki、R. Okano、N. Akiyama 和 H. Sugita：“日本比目鱼养殖水族馆微生物群落的抗菌能力”Suisanzoshoku。