Regulation of the Synthesis and assembly of the phofosynthesis protein of Euglena

眼虫光合成蛋白的合成和组装调控

• 批准号: 09640795
• 负责人: OSAFUNE Tetsuaki
• 金额: $ 1.98万
• 依托单位: Nippon Sport Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640795/
• 关键词: Euglena; LHCP II; RuBisco; COS; RuBisCO; ゴルジ装置; 塩化カドミウム; LHCP II; 免疫電顕法; ユ-グレナ; 葉緑体形成過程

Precursors to the Euglena chlorophyll a/b binding protein (pLHCPII) and small subunit of ribulose-bis-phosphate carboxylase (pSSU) are transported in vesicles from ER to Golgi to chloroplast. Immunoelectron microscopy (IM) localizes SSU and LHCPII in greening cells to a membranous cytoplasmic osmiophilic structure (COS), the Golgi and chloroplast but never the nucleus or cytoplasm. Euglena cultured in the dark without aeration accumulate lipid. Upon aeration, lipid decreases, SSU accumulates and proplastids expand with limited prothylakoid formation. IM localizes SSU to the nucleus, COS, vacuolar structures and developing plastids of dark aerated cells. 15 C blocks Golgi to chloroplast transport inhibiting light induced chloroplast development and LHCPII accumulation. IM localizes LHCPII to the nucleus, COS, Golgi and plastids of 15 C cells. Western blotting demonstrates that pLHCPII levels are identical at 15 and 26 C while mature LHCPII accumulates slower and to lower levels at 15 C.Taken together, these experiments suggest that nuclear localization of chloroplast proteins is associated with disruption of normal plastid development. Chloroplast proteins are localized to the nucleus either by vesicle mistargeting or as is more likely through disruption of chloroplast integrity, release of chloroplast proteins to the cytoplasm and their subsequent diffusion into the nuclear compartment.

裸藻叶绿素a/B结合蛋白（pLHCP II）和核酮糖二磷酸羧化酶（pSSU）的前体在囊泡中从内质网转运到高尔基体再到叶绿体。免疫电子显微镜（IM）定位SSU和LHCP II在绿化细胞的膜质嗜锇结构（COS），高尔基体和叶绿体，但从来没有细胞核或细胞质。裸藻在无通气的黑暗条件下培养，可积累油脂。通气后，脂质减少，SSU积累，前质体膨胀，形成有限的类原囊体。IM将SSU定位于暗通气细胞的细胞核、COS、液泡结构和发育中的质体。15 C阻断高尔基体向叶绿体的运输，抑制光诱导的叶绿体发育和LHCP II积累。IM将LHCP II定位于15 C细胞的细胞核、COS、高尔基体和质体。Western印迹表明，pLHCPII水平是相同的，在15和26 C，而成熟的LHCPII积累较慢，并在15 C较低的水平。两者合计，这些实验表明，叶绿体蛋白质的核定位与正常质体发育的破坏。叶绿体蛋白质定位于细胞核，或者通过囊泡错误启动，或者更可能通过破坏叶绿体完整性，将叶绿体蛋白质释放到细胞质中并随后扩散到核区室中。

项目成果

Ito-Kuwa, S.: "Oxidative stress sensitivity and superoxide dismutase of a wild type parent strain and a resporatory mutant of Candida albicans"Medical Mycology. 37. 307-314 (1999)

Ito-Kuwa, S.：“野生型亲本菌株和白色念珠菌呼吸突变体的氧化应激敏感性和超氧化物歧化酶”医学真菌学。

Mulholland, M.R.: "Nitrogen utilization and metabolism relative to patterns of N_2 fixation in cultures of Trichodesmium NIBB1067."J.Phycol.. 977-988 (1999)

Mulholland, M.R.：“与 Trichodesmium NIBB1067 培养物中 N_2 固定模式相关的氮利用和代谢。”J.Phycol.. 977-988 (1999)

長船哲斉 他2名: "環境・生命の科学"弘学出版. 87 (2000)

Tessai Osafune等2人：“环境与生命的科学”Kogaku Publishing 87（2000）。

Osafune, T.: "Immunocytochemical localization of RuBisCO in dark-grown wax-rich cells of Euglena : Dark synthesis of a novel large subunit of the enzyme outside the plastid"Electron Microscopy. 41-42 (2000)

Osafune, T.：“RuBisCO 在暗生长的富含蜡的眼虫细胞中的免疫细胞化学定位：质体外酶的新型大亚基的暗合成”电子显微镜。

Ohki, K.: "A possible role of temperate phage in the regulation of Trichodesmium biomass."In : Marine Cyanobacteria and Related Organisms (eds.Charpy, L.& Larkum) Bulletin de l'Institut Oceanographique Monaco, special. 19. 287-292 (1999)

Ohki, K.：“温带噬菌体在调节 Trichodesmium 生物量中的可能作用。”载于：海洋蓝藻和相关生物（eds.Charpy, L.