Immunocytochemical studies on the behavior of LHCP II in wax-rich cells of Euglena.

用抗LHCP II抗体和蛋白A-GOLD对纤细眼虫富蜡细胞进行免疫电子显微镜观察，发现LHCP II首先出现在Cos、高尔基体和高尔基体衍生的囊泡中，通过叶绿体的类囊体膜。高尔基体产生的囊泡与裸眼藻质体最外层的膜融合。在0.1%的链霉素存在下，叶绿体中的前板层小体(PLB)的消失受到抑制，在PLB和类蛋白核附近形成一个与LHCP II抗体呈免疫反应的类晶体小体，而类囊体中没有这种免疫反应。因此，在充气的富蜡细胞中的条件允许LHCP II脱辅基蛋白在链霉素上异常积累，阻止了这种脱辅基蛋白向类囊体体的分布。

Immunoelectron microscopy of wax-rich cells of Euglena gracilis Z using anti-LHCP II antibody and protein A-gold shows LHCP II first in the Cos, Golgi body and Golgi-derived vesicle by the thylakoids of the plastid. Golgi-derived vesicle fuses with the outermost membrane of the Euglena plastid. In the presence of 0.1% Streptomycin have plastids in which the disappearance of the prolamellar body (PLB) is inhibited ; a paracrystalline body is formed in close proximity to the the PLB and pyrenoid which shows an immunoreaction with LHCP II antibody, but the immunoreaction is absent from the thylakoids. Thus conditions in the aerated wax-rich cells allow an unusual accumulation of LHCP II apoprotein at Streptomycin blocks the distribution of this apoprotein to the thylakoids.