Immunocytochemical Studies on the Behavior of LHCP II during Chloroplast Development of Euglena Graclis.

细小眼虫叶绿体发育过程中 LHCP II 行为的免疫细胞化学研究。

• 批准号: 01540582
• 负责人: OSAFUNE Tetsuaki
• 金额: $ 1.22万
• 依托单位: Tokyo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540582/
• 关键词: Euglena; Golgi Apparatus; LHCP II; Chloroplast Development; Immunogold Localization; Euglena gracilis; LHCPII; Golgi apparatus

Anti LHCP II 26.5 kDa apoprotein antibody and protein A-gold were used to localize the apoprotein in darkーgrown resting cells of Euglena gracilis var. Bacillaris and Z strain exposed to 150 ft-c of light to induce normal chloroplast development. An antibody reaction appeared first in the Golgi apparatus (at about 12 hr of light exposure) and as the reaction in the Golgi increased, deposition was seen over the plastid thylakoids. By 48 hr of light exposure both Golgi and thylakoids showed a strong reaction. There was no reaction in Golgi or thylakoids, however, when preimmune serum was substituted for antiserum. By 72-96 hr of light exposure, reaction in the thylakoids was maximal but disappeared completely from the Golgi. The results suggest, in agreement with data from synchronous cells and light-intensity shift-up experiments, that the nascent LHCP-apoprotein passes through the Golgi and may be processed there, on the way to deposition in the thylakoids. An antibody reaction was detected in the compartmentalized osmiophilic body (COS) before it was seen in Golgi or thylakoids.

抗LHCP II 26.5 kDa载脂蛋白抗体和蛋白A-gold被用来定位载脂蛋白在黑暗的生长的静止细胞的眼虫gracilis变种。Bacillaris和Z菌株暴露于150 ft-c的光以诱导正常的叶绿体发育。抗体反应首先出现在高尔基体中（在约12小时的光照下），随着高尔基体中反应的增加，在质体类囊体上观察到沉积。光照48小时，高尔基体和类囊体都表现出强烈的反应。然而，当免疫前血清代替抗血清时，高尔基体或类囊体中没有反应。在72-96小时的光照下，类囊体中的反应是最大的，但从高尔基体完全消失。结果表明，在协议的数据从同步的细胞和光强度转移的实验，新生的LHCP-载脂蛋白通过高尔基体，并可能在那里处理，沉积在类囊体的方式。在高尔基体或类囊体中观察到抗体反应之前，在隔室化的嗜锇体（COS）中检测到抗体反应。

项目成果

Tetsuaki OSAFUNE: "Immunolocalization of LHCP II apoprotein in the Golgi during lightーinduced chloroplast development in nonーdividing Euglena cells," Journal of Electron Microscpy. 40. 41-47 (1991)

Tetsuaki OSAFUNE：“非分裂眼虫细胞光诱导叶绿体发育过程中 LHCP II 脱辅基蛋白的免疫定位”，电子显微镜杂志 40. 41-47 (1991)。

Tetsuaki Osafune,Tomoko Ehara,Shuji Sumida, Akira Ito,Eiji Hase.: "Incorporation of cytoplasmic structures by developing proplastids in dark-grown Euglena gracilis cells transferred to an inorganic medium in darkness." Journal of Electron Microscopy. 39.

Tetsuaki Osafune、Tomoko Ehara、Shuji Sumida、Akira Ito、Eiji Hase：“通过在黑暗中生长的眼虫细胞中发育原生质体来整合细胞质结构，并将其转移到黑暗中的无机介质中。”

Osafune, T., Ehara, T., Tahara, M., and Hase, E.: "Effects of blue and red light on chloroplast development in dark-grown wax-rich cells of Euglena gracilis transferred to inorganic medium." J. Electron Microscopy. 39(4). 254-259 (1990)

Osafune, T.、Ehara, T.、Tahara, M. 和 Hase, E.：“蓝光和红光对转移到无机介质中的眼虫黑暗生长的富含蜡细胞的叶绿体发育的影响。”

Ehara, T., Osafune, T., and Hase, E.: "Interaction between the nucleus and cytoplasmic organelles during the cell cycle of Euglena gracilis in synchronized culture IV. An aggregate form of chloroplasts in association with the nucleus." Exp. Cell Research.

Ehara, T.、Osafune, T. 和 Hase, E.：“同步培养 IV 中眼虫细胞周期期间细胞核和细胞质细胞器之间的相互作用。与细胞核相关的叶绿体的聚集形式。”

Osafune, T., Schiff, J. A. and Hase, E.: "Stage-dependent localization of LHCP II apoprotein in the Golgi of synchronized cells of Euglena gracilis by immunogold electron microscopy." Experimental Cell Research. 193(1).

Osafune, T.、Schiff, J. A. 和 Hase, E.：“通过免疫金电子显微镜观察眼虫同步化细胞高尔基体中 LHCP II 脱辅基蛋白的阶段依赖性定位。”