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Novel Binding Structures and Reactions of Metal Complexes of Some Combired Ligands

一些组合配体的金属配合物的新型结合结构和反应

基本信息

批准号：
09640674
负责人：
CHIKIRA Makoto
金额：
$ 0.7万
依托单位：
CHUO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

In this study, metal complexes of 1) bleomycin (Blm) and deglycopepromycin (Dpe), 2) acridine-polyamin, 3) histidine-containing peptides, 4) vilogen-porphyrin have been investigated on the novel binding structures and the reactions on DNA. The results are listed below.1) Fe(III)Blm binds DNA with two different orientations and NO in ON-Fe(II)Blm is held rigidly in place and the motion is severely restricted on the DNA. The low-spin Fe(III)Dpe changes irreversibly to high-spin species on DNA. Cu(II)Dpe cannot bind stereo specifically on DNA.2) Some platinum complexes of acridine-polyamines show more intense cytotoxicity than cis-platin. For the copper(II) complexes, it was found by DNA-fiber ESR spectroscopy that the coordinating moiety of some of the complexes is moving vigorously at room temperature.3) Cu(II) complex of L-histidine forms an aggregate stereospecifically bound on DNA. Cu(II) complexes of XィイD2aaィエD2-Gly-His (XィイD2aaィエD2=Gly, Lys, Arg) binds in the minor groove of B-form DNA. The sequence specific cleaving reactions by corresponding Ni(III) complexes were discussed in terms such a binding structure.4) Cu(II) complex of a meso-substituted tetra-viologen porphyrin binds DNA so strongly that it bridges double helical DNA, while the mono-substituted porphyrin complex is so unstable that it decomposes on DNA to form a Cu(II) complex with the unknown fragment derived from the porphyrin.The above results have been published in several journals or presented at the international and domestic symposiums as shown in the Research Report.

本研究研究了1)博来霉素（Blm）和去糖原霉素（Dpe）、2)吖啶-多胺、3)含组氨酸肽、4)紫绿素-卟啉等金属配合物在DNA上的新型结合结构和反应。结果如下所示。1) Fe(III)Blm以两种不同的方向结合DNA， NO在on -Fe(II)Blm被牢牢地固定在原位，在DNA上的运动受到严重限制。低自旋Fe(III)Dpe在DNA上不可逆地转变为高自旋物种。Cu(II)Dpe不能在dna上特异性结合立体。2)某些吖啶多胺铂配合物的细胞毒性比顺铂更强。对于铜（II）配合物，dna纤维ESR谱分析发现，一些配合物的配位部分在室温下剧烈移动。3) l -组氨酸的Cu（II）络合物在DNA上形成立体特异性结合的聚集体。铜(II)配合物的XィイD2aaィエD2-Gly-His (XィイD2aaィエD2 = g,赖氨酸,Arg)的小沟结合b形式的DNA。根据这种结合结构讨论了相应的Ni（III）配合物的序列特异性裂解反应。4)中位取代的四紫卟啉的Cu（II）配合物与DNA的结合非常强，可以桥接双螺旋DNA，而单取代的卟啉配合物非常不稳定，可以在DNA上分解，与卟啉衍生的未知片段形成Cu（II）配合物。以上研究成果已在多家期刊发表或在国内外学术会议上发表，详见研究报告。