Development of Rapid System for Ion-pair Reversed-phase Partition Mode HPLC by Using Hydrophilic Counter ion

利用亲水抗衡离子的离子对反相分配模式高效液相色谱快速系统的开发

基本信息

批准号：
09555261
负责人：
YOTSUYANAGI Takao
金额：
$ 5.44万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

A new method for HPLC with ion-pair reversed-phase partition mode has been developed by using hydrophilic counter ion such as buffer components and alkaline metal ion. The advantages of the proposed method are rapid elution and efficient separation. A marked improvement was attained compared with the conventional method by using hydrophobic counter ion such as quarternary ammonium ion and long chain alkylsulfonic ion.For example, anionic metal chelates with 2,2'-dihydroxyazobenzene resulted in base-line resolution within 6 min, In the conventional method with tetrabutylammonium on, it look 30 mm to separate these chelates. Furthermore, the proposed method has been successfully extended to the anionic metal chelates with 4-(2-pyridylazo) resorcinol and its analogues, polyaminocarboxylic acid such as Quin-2, and hydorazone derivatives. Compared to the conventional method, a significant improvement was achieved in retention time. The separation efficiency was comparable to that with tetrabutylammonium ion.It has been found that alkaline metal ions such as sodium, lithium and cesium ion, ammonium ion such as tetramethylammonium ion, and guanidium ion effectively work as the hydrophilic counter ion. The mechanism of the rapid retention is attributed to the size and hydration of the hydrophilic ion which leads to specific characteristics of the ion-pairs.The underlying retention mechanism of the charged anlyte in the proposed method with hydrophilic counter ion is also based on the partition of ion-pairs similar to that of hydrophobic counter ion. The proposed method serves as a innovative system for HPLC with ion-pair reversed-phase partition mode.

通过使用亲水性计数器（例如缓冲液组件和碱性金属离子），已经开发了一种具有离子对相反相分配模式的HPLC的新方法。该方法的优点是快速洗脱和有效的分离。与常规方法相比，通过使用疏水剂离子（例如季铵离子和长链烷基磺化离子）进行了显着改善。例如，在6分钟内，在6分钟内，在6分钟内，在6分钟内，用2,2'-二羟基苯苯在6分钟内，通过跨层群的分离，它可以分开，从而在6分钟内通过分离的30次分别进行基础方法，从而使以下30次分离。此外，提出的方法已成功扩展到具有4-（2-吡啶基唑）异醇及其类似物，聚氨基羧酸（例如Quin-2）和羟基酮衍生物的阴离子金属螯合物。与常规方法相比，保留时间取得了显着改善。分离效率与四丁酰铵离子相当。它已经发现碱金属离子（例如钠，锂和铯离子），铵离子，例如四甲基铵离子，而鸟苷离子有效地起到了水生酚反离子的有效作用。快速保留的机制归因于亲水离子的大小和水合，从而导致离子对的特定特性。在拟议的方法中，带有亲水反离子的拟议方法中，带电的ANLYTE的基本保留机制也基于与水生电鸡相似的离子对分配。提出的方法是具有离子对相反相分区模式的HPLC的创新系统。