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Development of Rapid System for Ion-pair Reversed-phase Partition Mode HPLC by Using Hydrophilic Counter ion

利用亲水抗衡离子的离子对反相分配模式高效液相色谱快速系统的开发

基本信息

批准号：
09555261
负责人：
YOTSUYANAGI Takao
金额：
$ 5.44万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

A new method for HPLC with ion-pair reversed-phase partition mode has been developed by using hydrophilic counter ion such as buffer components and alkaline metal ion. The advantages of the proposed method are rapid elution and efficient separation. A marked improvement was attained compared with the conventional method by using hydrophobic counter ion such as quarternary ammonium ion and long chain alkylsulfonic ion.For example, anionic metal chelates with 2,2'-dihydroxyazobenzene resulted in base-line resolution within 6 min, In the conventional method with tetrabutylammonium on, it look 30 mm to separate these chelates. Furthermore, the proposed method has been successfully extended to the anionic metal chelates with 4-(2-pyridylazo) resorcinol and its analogues, polyaminocarboxylic acid such as Quin-2, and hydorazone derivatives. Compared to the conventional method, a significant improvement was achieved in retention time. The separation efficiency was comparable to that with tetrabutylammonium ion.It has been found that alkaline metal ions such as sodium, lithium and cesium ion, ammonium ion such as tetramethylammonium ion, and guanidium ion effectively work as the hydrophilic counter ion. The mechanism of the rapid retention is attributed to the size and hydration of the hydrophilic ion which leads to specific characteristics of the ion-pairs.The underlying retention mechanism of the charged anlyte in the proposed method with hydrophilic counter ion is also based on the partition of ion-pairs similar to that of hydrophobic counter ion. The proposed method serves as a innovative system for HPLC with ion-pair reversed-phase partition mode.

利用缓冲液组分和碱金属离子等亲水性反离子，建立了一种离子对反相分配高效液相色谱新方法。该方法的优点是洗脱速度快，分离效果好。与传统方法相比，采用疏水性反离子季铵离子和长链烷基磺酸根离子，如2，2 '-二羟基偶氮苯与阴离子金属螯合物在6 min内实现基线分离，而采用四丁基铵离子的传统方法，这些螯合物的基线分离距离为30 mm。此外，所提出的方法已成功地扩展到阴离子金属螯合物与4-（2-吡啶偶氮）间苯二酚及其类似物，聚氨基羧酸，如Quin-2，和hydorazone衍生物。与传统方法相比，保留时间得到了显著改善。已经发现碱金属离子如钠、锂和铯离子、铵离子如四甲基铵离子和胍离子有效地作为亲水性抗衡离子起作用。快速保留的机理是由于亲水性离子的尺寸和水合作用导致离子对的特殊性，而在所提出的方法中，带电分析物与亲水性反离子的潜在保留机理也是基于类似于疏水性反离子的离子对分配。所提出的方法作为一个创新的系统与离子对反相分配模式的HPLC。