Analysis of aging-associated genes in myocardium : application of RT-PCR differential display method.

这是众所周知的，因为心脏的生理功能是被改变为衰老的。例如，异丙二烯醇和内替林-1的积极性与异丙二烯-1被衰老在大鼠肠道中降解。当B-肾上腺素受体的绑定能力被消除时，内皮素-1的特定绑定能力被增加在老年大鼠中。在sarcoplasmic reticulum中，Ca-y D12+ y D1的活动减少了。在冠状动脉内氮氧化物和硝酸氧化物的生产是非常低的老年大鼠。“这些年龄相关功能性变化的分子基础并没有被理解。”差异基因表达提供了与差异、开发和老龄化相关的许多基本细胞过程的基础。The Identification and cloning of genes whose expression is an important step in advancing our unstanding of aging phenomena。在订购分离和表征基因产品时，在老龄化中表现出不同的差异，我们应用了差异 ... More 线性显示反向转录-聚合酶链反应在2-3个月的旧和27个月的老Fischer 344只大鼠ventricles中筛选出这些基因。总的RNAs是由酸-guanidium-phenol-chloroform方法隔离的。信使RNA从使用寡核苷酸d（T）列的总RNA中获得。来自每个RNA的第一链cDNA与三个寡-d（T）-锚定引物合成。PCR用锚引物和八个算术引物携带，并且对序列凝胶进行了分析。19种独特的PCR产品显示了在衰老的心脏中增加的表达，而66种克隆人在衰老的心脏中减少。不同的表达式是由北方浮标分析或反向北方浮标证实的。克隆人包括用于线粒体代谢的基因，如细胞色素c氧化酶、myosin heavy chain、细胞表面糖蛋白质和多种激酶/磷酸酶等信号转导。31个克隆人被归类，14个克隆人被归类为未知基因。在本研究中使用的方法是有价值的，对于研究与年龄相关的基因表达，我们将继续分析在本研究中识别的未知基因。Less（低）

It has been known that the physiological function of the heart is altered according to the aging. For example, the positive inotropic effects of isoproterenol and endothelin-1 are decreased by aging in the rat ventricles. While the binding capacity for b-adrenergic receptor was decreased, the specific binding capacity of endothelin-1 was increased in the aged rats. In sarcoplasmic reticulum, the activity of CaィイD12+ィエD1 was reduced. In coronary arterial endothelium, nitric oxide production was very low-in aged rat. The molecular basis of these age-dependent functional changes has not been understood. Differential gene expression provides the basis for many fundamental cellular processes associated with differentiation, development and aging. The identification and cloning of genes whose expression is modulated is an important step in advancing our understanding of aging phenomena. In order to isolate and characterize gene products differentially expressed in aging, we applied the diffe … More rential display reverse transcription-polymerase chain reaction for screening those genes in 2-3 months-old and 27 months-old Fischer 344 rat ventricles. Total RNAs were isolated by acid-guanidium-phenol-chloroform method. Messenger RNAs were obtained from total RNA by using oligo d(T) column. The first-strand cDNAs from each RNA were synthesized with three oligo-d(T)-anchor primers. PCR was carried out with anchor primers and eight arbitrary primers, and the products were analyzed on sequence gels. The 19 unique PCR products displayed increased expression in aged heart, while 66 clones were decreased in aged heart. Differential expression was confirmed by Northern blot analysis or reverse Northern blotting. The clones included genes for mitochondrial metabolism such as cytochrome c oxidase, myosin heavy chain, cell surface glycoproteins and several kinases/phosphatases for signal transduction. 31 clones were sequenced and 14 clones were unknown genes. The method used in this study is valuable for studying the age-associated gene expression, and we are continuing to analyze the unknown genes identified in this study. Less