Forensic molecular biological studies of sex chromosomal DNA for the application to criminal and civil identification

性染色体 DNA 的法医分子生物学研究在刑事和民事鉴定中的应用

• 批准号: 09557039
• 负责人: ISHIZU Hideo
• 金额: $ 7.36万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557039/
• 关键词: Genetic polymorphism; Sexidentification; Sex chromosome; Microsatellite; Alu sequence; Amelogenin gene; Personal identification; Paternity testing

1. Sex determination by detection of Y chromosome-specific aiphoid repeat sequenceWe have established the conditions for sensitive amplification of the Y chromosome-specific aiphoid repeat sequence by PCR, and succeeded sex determination from a hair shaft of 2 cm in length.2. Detection of Y chromosomal microsatellite polymorphismsThe allele frequency of a microsatellite DYS 19 locus was estimated to be A=0.064, B=0.068, C=0.423, D=0.226, E=0.211, F=0.004 and C-2=0.004 (N=263) in Japanese living in Okayama and Hiroshima Prefectures. In these alleles, a C-2 allele, migrated between B and C alleles on electrophoretic gels, have not yet been reported in all the races. By the examination of family samples, the usefulness of this STR system in paternity tests was confirmed. Moreover, this STR system was useful for the identification of an assailant/assailants of a sexual assault. The other STR loci on the Y chromosome, DYS 390, DYS391 and DYS395 were also suggested to be useful in paternity tests and in sexual assault cases.3. Detection of an X chromosome microsatellite HPRT polymorphismThe allele frequency of a microsatellite at HPRT locus on the X chromosome was estimated to be T1=0.105, T24=0.243, T3=0.396, T4=0.200 and T5=0.057 (male=84, female=63) in Japanese living in Okayama Prefecture. This STR locus was suggested to be useful in paternity tests.4. Detection of the amelogenin geneWe have examined PCR products from various animals including human male and female amplified using primers for a human amelogenin sequence by restriction enzyme analysis and sequencing, and it was suggested that discrimination between those animals could be possible except that the animals were close phylogenetically. Moreover, using fluorescent dye-labeled primers, human male and female could be determined with high sensitivity by PCR.

1。通过检测Y染色体特异性烷烃重复序列确定性别，确定了通过PCR对Y染色体特异性的苯甲酸重复序列的敏感性放大的条件，并从长度为2 cm的头发轴成功地确定了性别确定。2。检测Y染色体微卫星多态性的检测微卫星Dys 19基因座的等位基因频率为A = 0.064，B = 0.068，C = 0.423，d = 0.226，d = 0.226，E = 0.211，F = 0.004和F = 0.004和C-2 = 0.004和C-2 = 0.004（n = 263），在日本中均为prefture and ossemama和hirosh and。在这些等位基因中，在所有种族中尚未报道过电泳凝胶上B和C等位基因之间的C-2等位基因。通过对家庭样本的检查，该STR系统在亲子鉴定中的有用性得到了证实。此外，该STR系统对于确定性侵犯的袭击者/袭击者很有用。还建议在Y染色体上的另一个STR基因座，DYS 390，DYS391和DYS395在亲子鉴定和性侵犯病例中有用。3。估计检测X染色体X染色体微卫星HPRT多态性的等位基因在X染色体上HPRT基因座的等位基因频率为T1 = 0.105，T24 = 0.243，T3 = 0.396，T3 = 0.396，T4 = 0.200和T4 = 0.200和T5 = 0.200和T5 = 0.057（男性= 84 = 84，perfuctive coopertucation nive in fike 63）。该基因座被认为对亲子鉴定有用4。通过限制酶的分析和测序，发现了氨基蛋白酶Genewe的检测已检查了来自各种动物的PCR产物，包括使用引物进行人氨基蛋白素序列的引物扩增的PCR产物，并且建议可以在这些动物之间进行歧视，除了这些动物是近距离的。此外，使用荧光染料标记的底漆，可以通过PCR高灵敏度确定人类和女性。