STUDIES ON MUSCLE-ASSOCIATED SUBSTANCES FOR THE MEDICO-LEGAL DIAGNOSIS

研究了法医标本中肌肉相关物质（MAS）的含量。心肌肌钙蛋白T （TnT）含量在研究病例间存在差异，且与心肌肌钙蛋白T及肌红蛋白（Mb）浓度无相关性。骨骼肌中MAS的含量在不同病例和19个部位之间也存在差异。因此，肌肉中MAS含量对法医尸检的法医学诊断的有用性尚不能得到证实。中枢/动脉血中TnT和心肌肌钙蛋白I的浓度高于外周/静脉血。骨骼肌中CPK和肌酸含量高于心肌，外周血/静脉血中CPK和肌酸含量高于外周血/动脉血。下腔静脉血中GOT和GPT活性高于其他部位血。这些结果可能阐明在中毒情况下MAS或药物的死后循环和扩散状态。当从法医学角度考察血液或其他体液中MTAS浓度时，左右心血中TnT和TnI浓度可作为诊断溺水的补充参数。心包液肌酐浓度、脑脊液和玻璃体体液肌酐浓度也可用于估计死后时间。通过检测Mb基因第3外显子的人类特异性序列，可以从法医生物材料中鉴定出Mb基因的人类起源。Mb基因第2外显子snp的分析为法医鉴定和亲子鉴定提供了依据。

The contents of muscle-associated substances (MAS) in the forensic specimens were investigated. The content of cardiac troponin T (TnT) in myocardium was different among the investigated cases and had no correlation with the concentration of this protein in cardiac blood, as well as that of myoglobin (Mb). The contents of MAS of skeletal muscle were also different among cases and 19 sites investigated. Therefore the usefullness of the contents of MAS in muscle for medico-legal diagnosis in forensic autopsy could not be proved. Concentration of TnT and cardiac troponin I was higher in central/arterial blood than in peripheral/venous blood. CPK and creatine, that are more contained in skeletal muscle than in myocardium, were determined more in peripheral/venous blood than in central/arterial blood. Activities of GOT and GPT in the blood of vena cava inferior was higher than in blood obtained form any other site. These results may elucidate the state of postmortem circulation and diffusion of MAS or drugs in case of intoxication. When the MTAS concentration in blood or other body fluids was investigated from medico-legal aspects, the TnT and TnI concentrations in right and left heart blood were useful as a supplemental parameters to diagnose drowning. The creatinine concentration in pricardial fluid and the creatine concentrations in cerebrospinal fluid and vitreous humor were also useful for estimating postmortem interval. By detection of the human-specific sequence in the 3rd exon of Mb gene it was possibe to identify the human origin from forensic biological materials. The analysis of SNPs in the 2nd exon of Mb gene were useful for forensic personal identification and paternity test.