Molecular analysis of Genetic Polymorphisms in Blood and Development of Primers for Genotyping

在这个项目中，我们研究了经典遗传标记物的分子基础，以建议在血液中使用简单和快速的基因型方法。我们分析了（1）ABO、RH和Diego血液组系统，如红细胞抗原， （2）类风湿体（Alpha-1-酸糖蛋白）、组特异性成分、Alpha-trypsin抑制剂、第一成分的R亚成分、补充的第七成分、Alpha-1-抗肽和结合因子XIII的B单位如血清蛋白（3）磷酸化酶1、eaterase D、DNase 1，乙酰脱氢酶2和磷酸酶2分别是红细胞和其他细胞酶。We also analyzed some null alleles （ORM1イD1* イD1Q0koln and ITIH1イD1* イD1Q0iwate）， which could cause a false incompatibility between father and child。一个新突变的核肽替代品，在一个德国遗传性案件中观察到的群体特定成分，已确定。在这一研究中发现的发现将不仅仅是在法医科学中，但也是生物学和医学科学中。

In this projects we have investigated the molecular bases of classical genetic markers in blood to propose simple and rapid genotyping methods. We analyzed (1)ABO, RH and Diego blood group systems as red cell antigens, (2)orosomucoid (alpha-1-acid glycoprotein), group-specific component, inter-alpha-trypsin inhibitor, the R subcomponent of the first component of component, the seventh component of complement, alpha-1-antitrypsin, and the B unit of coagulation factor XIII as serum proteins (3)phosphoglucomutase 1, eaterase D, DNase 1, acetaldehyde dehydrogenase 2, and phosphomannomutase 2 as red cell and other cell enzymes. We also analyzed some null alleles (ORM1ィイD1*ィエD1Q0koln and ITIH1ィイD1*ィエD1Q0iwate), which could cause a false incompatibility between father and child. A nucleotide substitution of de novo mutation, observed in the group-specific component in a German paternity case, was determined. The findings obtained in this study will be useful not only in forensic science but also biological and medical sciences.