The preventive mechanisms of life-style related disease by edible plant extracts and their application for the disease

食用植物提取物预防生活方式相关疾病的机制及其在疾病中的应用

• 批准号: 16500515
• 负责人: YUASA Isao
• 金额: $ 1.98万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500515/
• 关键词: Edible plant extracts; Life style-related disease; Antitumor activity; Dietary fiber; Tea; Active oxygens; Cell Culture; 細胞レベル; 月見草抽出物; 活性酸素; 細胞増殖; アポトーシス; ガン細胞; 活性酸素種; ポリアミン; 細胞周期; ニンジン抽出物

The purposes of this study are following. One is to understand the mechanisms of antitumor activity of extracts of Aloe, green tea, evening primrose and others. Another is to examined the effect of combined treatment of these extracts.On the mechanism of antitumor activity of evening primrose extract in Ehrlich ascites tumor cells, we demonstrated that evening primrose extract (EPP) induced apoptosis and inhibited the DNA synthesis in Ehrlich ascites tumor cells (EATC) and suggested that EPE-induced inhibition of the growth of EATC are via at least two pathway differentially modulated by reactive oxygen species, notably intracellular peroxides. These are (a) the EPE-induced apoptosis pathway which is dependent on increases in hydrogen peroxide and (b) the EPE-induced inhibition of cell proliferation which is hydrogen peroxide independent. In this study EPE brought about a significant decrease in intracellular polyamine levels. Furthermore, the addition of polyamines reversed the EPE-induced decrease in cell viability and suppressed the EPE-induced increase in intracellular hydrogen peroxide. However, the addition of polyamines did not reverse EPE-induced decrease in DNA synthesis and phosphorylation of Rb protein, and EPE-induced translocation of AIF. These results suggest the involvement of polyamines in EPE-induced apoptosis pathway which is dependent on increase in hydrogen peroxide. We also examined the protective effect of green tea extract and tea polyphenols against FK-506-induced cytotoxicity in renal cells. The nephrotoxicity induced by the immunosuppressive drug FK506 limits its usefulness in widespread application.

本研究的目的如下。一是了解芦荟、绿色茶、月见报春花等提取物的抗肿瘤作用机制。另一个是研究这些提取物的联合治疗作用。关于月见草报春花提取物对埃利希腹水瘤细胞的抗肿瘤作用机制，我们证明了月见草报春花提取物（EPP）诱导埃利希腹水瘤细胞（EATC）凋亡并抑制DNA合成，并提示EPE-EATC生长的诱导抑制是通过至少两种由活性氧物质，特别是细胞内过氧化物差异调节的途径。这些是（a）依赖于过氧化氢增加的EPE诱导的细胞凋亡途径和（b）不依赖于过氧化氢的EPE诱导的细胞增殖抑制。（B）EPE诱导的细胞增殖抑制。在这项研究中，EPE带来了细胞内多胺水平的显着下降。此外，多胺的加入逆转了EPE诱导的细胞活力下降，并抑制了EPE诱导的细胞内过氧化氢的增加。然而，多胺的加入并没有逆转EPE诱导的DNA合成和Rb蛋白磷酸化的减少，以及EPE诱导的AIF易位。这些结果表明，多胺的参与在EPE诱导的细胞凋亡途径，这是依赖于过氧化氢的增加。我们还研究了绿色茶提取物和茶多酚对FK-506诱导的肾细胞毒性的保护作用。免疫抑制剂FK 506的肾毒性限制了其广泛应用。

项目成果

Protective effect of green tea extract and tea polyphenols against FK506-induced cytotoxicity in renal cells

• DOI: 10.1111/j.1742-7843.2006.pto_284.x
• 发表时间: 2006-02-01
• 期刊: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
• 影响因子: 3.1
• 作者: Hisamura, F;Kojima-Yuasa, A;Matsui-Yuasa, I
• 通讯作者: Matsui-Yuasa, I

免疫と栄養

免疫和营养

• 作者: 今大路治之;大岡唯祐;後藤恭宏;成谷宏文;堀内功典;鈴木基生;岡崎勝一郎;林哲也;桑原知己;間世田 英明 他;小倉裕範
• 通讯作者: 小倉裕範

トラーゲン産生向上剤

Tragen生产改良剂

• 发表时间: 2004

亜鉛欠乏による肝繊維化のメカニズム-肝細胞と肝星細胞の相互作用-

缺锌导致肝纤维化的机制 - 肝细胞与肝星状细胞的相互作用 -

• 发表时间: 2005
• 期刊: 生活科学研究誌 3
• 作者: 阪田麻友美;小島(湯浅)明子;本庄亜希子;湯浅勲
• 通讯作者: 湯浅勲

Screening of spice and herb extracts for cytotoxic activity against Ehrlich ascites tumor cells and DPPH radical scavenging activity

筛选香料和草药提取物对艾利希腹水肿瘤细胞的细胞毒活性和 DPPH 自由基清除活性

• 发表时间: 2004
• 期刊: ITE Letters 5
• 作者: Kametani;S.;Matsui-Yuasa;I.;Kojima-Yuasa;A.;Arimura;T.;Kennedy;D.O.;Honzawa;M.;Kikuzaki;H.;Nakatani;N.
• 通讯作者: N.