Development of a Raman Microspectrometer for Ultraviolet Excitation
紫外激发显微拉曼光谱仪的研制
基本信息
- 批准号:09554032
- 负责人:
- 金额:$ 7.74万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Raman microspectroscopy is a useful method to investigate the structures of micrometer-sized particles in a non-destructive manner. On the other hand, ultraviolet resonance Raman spectroscopy provides key information on the structures of proteins and nucleic acids. In this study, we have developed an ultraviolet Raman microspectrometer to exploit the advantages of both spectroscopic methods. The microspectrometer consists of a continuous-wave ultraviolet laser, an ultraviolet microscope, and a Raman spectrometer. The ultraviolet laser beam is obtained by frequency-doubling visible laser light from an Ar^+ laser. The ultraviolet microscope is specially designed for high throughput in the ultraviolet region and high spatial resolution of microscopic images by employing the confocal arrangement. Samples under a microscope objective are irradiated with ultraviolet laser light and the Raman scattered light was collected with the same objective and then guided to the Raman spectrometer. We have succeeded to record Raman spectra of proteins and nucleic acids by using the newly developed ultraviolet Raman microspectrometer. Detailed examination of the spectrometer performance has shown that the throughput of microscope objective is the most important factor that determined the quality of spectra to be obtained. To improve the performance of the microspectrometer, we are planning to design a new objective. The present research has established the basis for future development of an ultra-sensitive ultraviolet resonance Raman microspectrometer, which will be applicable to structural analysis of molecules and molecular assemblies even in living cells.
拉曼显微光谱学是一种无损研究微米级粒子结构的有效方法。另一方面,紫外共振拉曼光谱提供了蛋白质和核酸结构的关键信息。在这项研究中,我们开发了一种紫外拉曼显微光谱仪,以利用两种光谱方法的优点。微谱仪由连续波紫外激光器、紫外显微镜和拉曼光谱仪组成。紫外激光束是由来自Ar^+激光器的倍频可见激光获得的。紫外显微镜是专为高通量在紫外区和高空间分辨率的显微图像采用共聚焦排列。用紫外激光照射显微镜物镜下的样品,用同一物镜收集拉曼散射光,然后引导到拉曼光谱仪。我们利用新研制的紫外拉曼显微光谱仪成功地记录了蛋白质和核酸的拉曼光谱。对光谱仪性能的详细考察表明,显微镜物镜的通量是决定所得光谱质量的最重要因素。为了提高微谱仪的性能,我们计划设计一种新的物镜。本研究为未来研制超灵敏紫外共振拉曼显微光谱仪奠定了基础,该光谱仪将适用于活细胞中分子和分子组合的结构分析。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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TAKEUCHI Hideo其他文献
TAKEUCHI Hideo的其他文献
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{{ truncateString('TAKEUCHI Hideo', 18)}}的其他基金
Designguidelinefor frequencytunable terahertz electromagnetic wave emittersonthe basis of semiconductorphysics
基于半导体物理的频率可调太赫兹电磁波发射器设计指南
- 批准号:
22760010 - 财政年份:2010
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Structural Analysis of Biomolecular Assemblies by Flow Orientation in Micro-channels
通过微通道中的流动方向进行生物分子组装体的结构分析
- 批准号:
19350002 - 财政年份:2007
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Dynamic control of protein structure by cation-π interaction
通过阳离子-π相互作用动态控制蛋白质结构
- 批准号:
16350003 - 财政年份:2004
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structural Analysis of Membrane Proteins by UV Raman Linear Intensity Difference Spectroscopy
紫外拉曼线性强度差光谱法对膜蛋白的结构分析
- 批准号:
11440169 - 财政年份:1999
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Resonance Raman Microscopic Analysis of Hemoproteins in Living Cells
活细胞中血红素蛋白的共振拉曼显微镜分析
- 批准号:
09680643 - 财政年份:1997
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of an Ultraviolet Raman Microspectrometer Applicable to Structural Analyzes of Cells
适用于细胞结构分析的紫外拉曼显微光谱仪的研制
- 批准号:
07554025 - 财政年份:1995
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Ultraviolet Resonance Raman Spectroscopic Study on the Conformation of Enkephalin and Binding Mode to Receptors
脑啡肽构象及受体结合方式的紫外共振拉曼光谱研究
- 批准号:
01470142 - 财政年份:1989
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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