Involvement of the abnormality of cyclins, cyclin-dependent kinases or tumor suppressor gene products in the acquisition of the malignant potential of the endometrial carcinomas.

细胞周期蛋白、细胞周期蛋白依赖性激酶或抑癌基因产物的异常参与子宫内膜癌恶性潜能的获得。

基本信息

批准号：
09470355
负责人：
NIKAIDO Toshio
金额：
$ 9.15万
依托单位：
Shinshu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Interactions of cyclins, cyclin-dependent kinases (cdks) and tumor suppressor gene products are essential in cell cycle progression, and deranged expression of these factors are reported to evoke malignant transformation of the cell. To elucidate the tumorigenetic process of the endometrial carcinomas, expression of the cell cycle related molecules in normal and malignant endometrial tissues was investigated by immunostaining. In the normal endometrium, expression of cyclin A, cyclin B1, cyclin E, cdc2, cdk2 and p16 was sporadically observed in the glandular epithelia of the proliferative phase. In 64 cases of endometrial carcinomas, the number of positive cases for cyclin A, cyclin B1, cyclin D1, cyclin E, cdc2, cdk2, cdk4, p53, P16 and RB was 11, 32, 22, 41, 43, 32, 8, 22, 39, and 32 cases, respectively. The topographical distribution of cyclin D1, cyclin A and p53 positive cells correlated each other. Above 64 cases were classified into two groups. The first group was consisted of the cases showing positive for only cyclin E, and the second group was consisted of those expressing cyclin D1, p53 and cyclin A. The first group tended to contain well differentiated cases, and poorly differentiated cases were more frequently found in the second group. These findings suggest that accumulations of abnormalities of the cell cycle related factors are involved in the acquisition of the malignant potential of the endometrial carcinomas.

细胞周期蛋白，细胞周期蛋白依赖性激酶（CDKs）和肿瘤抑制基因产物的相互作用在细胞周期进程中至关重要，据报道这些因子的毁灭表达唤起细胞的恶性转化。为了阐明子宫内膜癌的肿瘤性过程，通过免疫染色研究了正常和恶性子宫内膜组织中细胞周期相关分子的表达。在正常子宫内膜中，细胞周期蛋白A，细胞周期蛋白B1，细胞周期蛋白E，CDC2，CDK2和P16的表达在增殖相的腺上皮中偶有地观察到。在64例子宫内膜癌病例中，细胞周期蛋白A，细胞周期蛋白B1，细胞周期蛋白D1，Cyclin E，CDC2，CDK2，CDK2，CDK4，P53，P16和RB的阳性病例数为11，32，22，22，22，41，43，43，43，32，32，32，32，32，32，8，8，8，22，39，32例。 Cyclin D1，Cyclin A和P53阳性细胞的地形分布相互关联。将64例以上的病例分为两组。第一组由仅显示细胞周期蛋白E阳性的病例组成，第二组由表达细胞周期蛋白D1，p53和细胞周期蛋白A的那些组成。第一组倾向于包含良好的差异化病例，而在第二组中更常见的病例更常见。这些发现表明，与细胞周期相关因素异常的积累与子宫内膜癌的恶性潜力的获取有关。