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The characteristics of amniotic membrane and it's application for regenerative medicine.

羊膜的特性及其在再生医学中的应用。

基本信息

批准号：
16390473
负责人：
NIKAIDO Toshio
金额：
$ 9.09万
依托单位：
University of Toyama (2005-2006)Shinshu University (2004)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

The subunits of laminin (α2, α3, αβ1, β3, γ1, γ2) were detected on basement membrane of the amnion. The RNAs, Oct4 (which is expressed on undifferentiated ES cells), Nestin or Musashi-1 (which is expressed on Neuronal stem cells), were expressed on amniotic cells.These results suggest that there were undifferentiated cells in amnion.The amnion can produce α1-antitrypsin (α1-AT) that inhibits the activity of trypsin or the activity of neutrophilic elastase in the amniotic fluid. The decreasing of α1-AT by inflammation or by infection, cause premature rupture of the membrane (PROM), and the PROM made the patient premature labor.This study showed that amniotic cells, 1) produce albumin or α1-AT as a parameter of hepatocyte, 2) produce the mRNA of Insulin and decreasing the blood glucose in diabetic model mouse, 3) express NKX2.5 (which is specific on cardiac muscle), ANP (which is specific on muscle of atrium), MHC (which is specific protein for contraction on cardiac muscle) by differentiation factor, 4) express mRNA of collagen Type II (which is specific protein on chondrocyte) under pellet culture with TGF-β3, BMP (which is the growth or differentiation factor for chondrocyte and osteocyte) and its receptors.5) hAE, which was seeded on the inner surface of artificial and porous tube, inhibited the invasion of fibroblast from outer area of tube, 6)Amniotic cell sheet was made by using the temperature sensitive polypeptide.There results suggest that amniotic cells are useful to regenerative medicine.

羊膜基底膜上可见层粘连蛋白α2、α3、αβ1、β3、γ1、γ2亚基。羊膜细胞表达Oct 4（在未分化的ES细胞上表达）、Nestin和Musashi-1（在神经干细胞上表达）等RNA，提示羊膜中存在未分化的ES细胞，羊膜可产生α1-抗胰蛋白酶（α1-AT），抑制羊水中胰蛋白酶或嗜酸性弹性蛋白酶的活性。本研究表明，糖尿病模型小鼠羊膜细胞可产生白蛋白或α1-AT，产生胰岛素mRNA，降低血糖，表达NKX2.5（对心肌有特异性），ANP（对心房肌有特异性）、MHC（其是心肌收缩的特异性蛋白）通过分化因子，4）表达II型胶原的mRNA TGF-β_3、BMP、TGF-β_3、BMP-β_4、BMP-（软骨细胞和骨细胞的生长或分化因子）及其受体。5）将hAE接种在人工多孔管的内表面，抑制成纤维细胞从管的外部区域侵入，6）利用温度敏感多肽制备羊膜细胞片，结果表明羊膜细胞可用于再生医学。