权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

FUNDAMENTAL STUDY AND APPLICATION OF OPTICAL CHROMATOGRAPHY - TRACE ANALYSIS OF BIOACTIVE SUBSTANCES AND PRECISE DETERMINATION OF MICROORGANISM'S POWER

光学色谱基础研究与应用-生物活性物质的痕量分析和微生物威力的精确测定

基本信息

批准号：
09450310
负责人：
IMASAKA Totaro
金额：
$ 9.15万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

A new method for separation of particles by optical radiation pressure, named as optical chromatography, was developed and applied to the determination of protein, microorganism's power, and deformability of erythrocyte. In order to evaluate the performance of optical chromatography, a number of equations are theoretically derived using a ray-optics model. Based on the results obtained by theoretical calculation, the dynamic range can be extended by increasing laser power and by decreasing flow rate. Optical chromatography was applied to trace analysis of protein. Two polystyrene beads, coated with antibody, are combined in the presence of an antigen. The bound (B) and free (F) beads were readily separated by optical chromatography, and the B/F ratio could be correlated with the concentration of antigen (protein). The rates of the forward and reverse immunological reactions were independently determined by measuring the time of formation and dissociation, respectively, of the immunobeads. The technique, called as optical funnel, utilized a system in which a microorganism is suspended in a flowing medium and radiation pressure, which is applied in the opposite direction. At equilibrium, the microorganism is captured. At this point, it has the capability to escape on its own power. Using this technique, it is possible to measure the magnitude of the microorganism's power. The optical funnel was also applied to the determination of the motility force of bovine sperm cells. The motility force of the sperm cell was measured in the aqueous solution at different pH values and potassium ion concentrations. It was possible to measure more than 250 sperm cells in three hours. Deformability of erythrocyte was evaluated by optical funnel using near infrared laser. Elongation of erythrocyte was measured as 2.4 ± 0.6 for young cells and 2.1 ± 0.5 for old cells, which were in good agreement with the values obtained by the other method.

开发了一种利用光辐射压分离颗粒的新方法——光色谱法，并将其应用于蛋白质、微生物能力、红细胞变形能力的测定。为了评估光学色谱的性能，使用射线光学模型从理论上推导了许多方程。根据理论计算的结果，可以通过增加激光功率和降低流量来扩大动态范围。光学色谱法用于蛋白质的痕量分析。两个涂有抗体的聚苯乙烯珠在抗原存在下结合。结合的 (B) 和游离的 (F) 珠子很容易通过光学色谱法分离，并且 B/F 比率可以与抗原（蛋白质）的浓度相关。通过分别测量免疫珠的形成和解离时间来独立确定正向和反向免疫反应的速率。该技术被称为光学漏斗，利用了一种系统，其中微生物悬浮在流动介质中，并在相反方向施加辐射压力。达到平衡时，微生物被捕获。到了这个时候，它已经有能力依靠自己的力量逃跑了。使用这种技术，可以测量微生物力量的大小。光学漏斗也被应用于测定牛精子细胞的运动力。在不同pH值和钾离子浓度的水溶液中测量精子细胞的运动力。三小时内可以测量超过 250 个精子细胞。使用近红外激光通过光学漏斗评估红细胞的变形能力。测量年轻细胞的红细胞伸长率为 2.4 ± 0.6，老年细胞的红细胞伸长率为 2.1 ± 0.5，这与其他方法获得的值非常一致。