Analyse der Sekundärmodifikationen von Komponenten der Phytochrom-Signaltransduktion in Arabidopsis thaliana

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Mapping of complex signal transduction networks has so far mainly relied on the analysis of mutants, studies of interactions in vitro and in vivo and on the biochemical characterisation of single components. Modern mass spectrometry allows the identification of single proteins and the characterisation of secondary modifications of peptides in complex mixtures. This allows a timeresolved analysis of the status of known signalling components. The signalling pathway of phytochrome A (phyA) which can specifically be triggered by far-red light thus uniquely allowing the observation of a single signalling chain with many known components will be targeted using the proposed technique. The project and a planned proposal with Baginsky (Halle) aim to detect spacial and temporal secondary modifications of key phyA signalling components in complex proteome samples and hence to elucidate the interplay of the key components with the help of SRM (selected reaction monitoring) mass spectrometry (LC-ESI-MS/MS).