Isolation of the rice DL gene by positional cloning.

通过定位克隆分离水稻 DL 基因。

• 批准号: 09460002
• 负责人: HIRANO Hiroyuki
• 金额: $ 8.7万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460002/
• 关键词: DL gene; positional cloning; Flower development; Reproduction organ; midrib; rice (Oryza sativa); Morphogenesis; carpel identity; ポジショナル クローニング; BACクローン; BAC クローン

The drooping leaf (dl) in rice (Oryza sativa) mutant shows homeotic conversion of carpels into stamens and drooping leaf without midrib. To elucidate the molecular function of the DL gene and to understand mechanism underlying flower development in rice, we have tried to isolate the DL gene by positional cloning. First, the dl locus was mapped within the RFIJP markers and the BAC contig covering about 250kb was constructed. Then, using a mutant probably caused by chromosomal deletion, the region involving the dl locus was confined within 50kb. Finally, the DL gene was isolated in combination with transposon tagging using TOS17. Sequence and Southern blot analyses revealed that eight dl alleles have mutations, of which severity was associated with that of their phenotypes. Therefore, we concluded that we succeeded in the isolation of the DL gene. The DL gene encodes a novel class of transcription factor, which is not found in any ABC genes so far reported. These findings suggest that the flower development in rice is regulated by the mechanisms distinct from that in Arabidopsis and Antirrhinum.

大米（Oryza sativa）突变体中下垂的叶子（DL）表明，在没有Midrib的情况下，地球转换为雄蕊和下垂的叶子。为了阐明DL基因的分子功能并了解水稻花的开发机制，我们试图通过位置克隆分离DL基因。首先，将DL基因座映射在RFIJP标记中，并构建了约250kb的BAC重叠群。然后，使用可能由染色体缺失引起的突变体，涉及DL基因座的区域被限制在50kb内。最后，将DL基因与使用TOS17结合使用转座子标记。序列和南部印迹分析表明，八个DL等位基因具有突变，其严重程度与其表型相关。因此，我们得出的结论是，我们成功地隔离了DL基因。 DL基因编码一种新型的转录因子，迄今为止的任何ABC基因都找不到。这些发现表明，大米的花朵发育受到与拟南芥和抗抗逆性不同的机制的调节。

项目成果

Kikuchi, K., M. Ueguchi-Tanaka, K. T. Yoshida, Y. Nagato, M. Matsusoka, and H.-Y. Hirano: "Molecular analysis of the NAC gene family in rice."MOL. Gen. Genet.. 262. 1047-1051 (2000)

Kikuchi, K.、M. Ueguchi-Tanaka、K. T. Yoshida、Y. Nagato、M. Matsusoka 和 H.-Y。

Hirano, H.-Y., and Y. Sano: "Enhancement of Wx gene expression and the accumulation of amylose in response to cool temperatures during seed development in rice."Plant Cell Physiol.. 39. 807-812 (1998)

Hirano, H.-Y. 和 Y. Sano：“水稻种子发育过程中 Wx 基因表达的增强和直链淀粉的积累响应低温。”植物细胞生理学.. 39. 807-812 (1998)

Mikami, I.: "Altered tissue gpecifre expression of the w<@D2x@>D2 gene of the opague mutations in rice"Euphyfica. 105. 91-97 (1999)

Mikami, I.：“水稻不透明突变的 w<@D2x@>D2 基因的组织 gpecifre 表达的改变”Euphyfica。

Hirano, H. -Y.: "Isolation of the DROOPING LEAF gene that regulates carpel development and snidrib formation in rice"Gamma Fild Symposia. 38(in press). (2000)

Hirano, H. -Y.：“分离调节水稻心皮发育和穗条形成的下垂叶基因”Gamma Field Symposia。

Kikuchi, K.: "Chromosomal location of the OsNAC gene." Rice Genet.Newslett.15 (in press). (1998)

Kikuchi, K.：“OsNAC 基因的染色体位置。”