Mechanism of nutrient supply in the metamorphosed stage of anomurans, particularly paralithods, and its application for the resource enhancement.

拟石蟹Paralithodescamtschaticus和短足拟石蟹Paralithodesbrevipes（十足目：异尾目：石蟹科：拟石蟹Paralithodes）是北太平洋和鄂霍次克海的重要经济帝王蟹。本研究首次阐明了白皮松在变质阶段不取食。(1)比较了蚤状幼体、蓝绿藻和幼体的口器。青冈的下颌骨，特别是侧叶的大小减少。颌突未钙化，缺乏致密性。上颌骨的内突和内足基本上有微小的刚毛。(2)灰绿苔类蚤状幼体前肠的刚毛减少，压滤机不发达。第一龄幼鱼的胃磨较发达，但没有。这些事实表明，蓝绿藻的进食和食物加工功能不起作用。新孵化的蚤状幼体在中肠腺的上皮细胞（吸收细胞）中具有脂质储备。脂质的积累是 ...更多信息 在所有的蚤状幼体阶段，在中肠腺的中间部分观察到饲养培养的硅藻海链藻和卤虫无节幼体。在早期的白皮松中观察到的大的脂滴在晚期的白皮松中消失。晚青冈中肠腺表上皮细胞外缘富含小脂滴。脂肪酸分析表明，中性和极性脂质含量从刚孵化的中华绒螯蟹蚤状幼体的41和84微克/毫克干重增加到最后的蚤状幼体和早期的蓝尾蚤状幼体的约100和110微克/毫克干重。脂含量在中期下降到60和80 μ g/mg，后期下降到25和50 μ g/mg，第1龄下降到15.30 μ g/mg。中性脂含量相对于极性脂含量有显著的增加/减少。这些事实表明，在非摄食阶段，脂被用作能源。在青冈中没有观察到存在于葛根血腔中的脂肪体。这可能意味着相对较短的距离迁移，在海岸招募过程中，在青杨属的长距离迁移相比。少

Paralithodes camtschaticus and P.brevipes (Decapoda : Anomura : Lithodidae : Paralithodes) are commecially important king crabs in the North Pacific Ocean and Okhotsuk Sea. The present study has elucidated, for the first time, that the glaucothoe, metamorphose stage, does not feed. (1) The mouthparts of zoea, glaucothoe and juveniles were compared. The mandibles of the glaucothe, especially the lateral lobes showed reduction in size. The gnathal processes are uncalcified, lacking denticles. The endites and endopods of the maxillae are rudimentally having minute setae. (2) The setae which presented in the foregut of zoea are reduced and the filter press is poorly developed in glaucothoe. The complex gastric mill is absent, which is well developed in the first juvenile. These facts suggest that feeding and food processing are not functional in the glaucothoe.The newly hatched zoea has lipid reserve in the epithelial cells (resorptive cells) of the midgut gland. Accumulation of lipids is … More observed in the medial portion of the midgut gland with feeding cultured diatom Thalassiosira sp. and Artemia nauplii during all zoea stages. The large lipid droplets obseved in the early glaucothoe disappeared in the late glaucothoe. Small lipid droplets distribute abundantly in the outer margin of the epithelial cells of the midgut gland tables in the late glaucothoe. Fatty acids analysis showed an increase of neutral and polar lipids contents at 41 and 84 ug/dry weight mg for the newly hatched zoea of P.camtschaticus to about 100 and 110 ug/mg at the final zoea and the early glaucothoe. The lipids content decreased to 60 and 80 ug/mg at the mid-stage glaucothoe, 25, 50 ug/mg at the late-stage, and 15.30 ug/mg at the first juvenile Neutral lipids content increased/decreased remarkably compared to polar lipids content. These facts indicate that the lipids are used as energy source during the non-feeding glaucothoe stage. The fat body, which presents in the hemocoel of the puerulus, was not observed in the glaucothoe. This may imply relatively short distance migration in glaucothoe compared to long distance migration in puerulus during coastal recruitment. Less