Mechanism of nutrient supply in the metamorphosed stage of anomurans, particularly paralithods, and its application for the resource enhancement.

异壁动物特别是拟石兽变态阶段的养分供应机制及其在资源增值中的应用。

• 批准号: 09460091
• 负责人: KITTAKA Jiro
• 金额: $ 0.83万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460091/
• 关键词: Paralithodes camtschaticus; Lithodidae; glaucothoe; mouthparts; midgut gland; fatty acid; neutral lipid; non-feeding; ハナサキガニ; 吸収細胞; 着底; 脂肪酸組成; EPA; 無摂餌段階

Paralithodes camtschaticus and P.brevipes (Decapoda : Anomura : Lithodidae : Paralithodes) are commecially important king crabs in the North Pacific Ocean and Okhotsuk Sea. The present study has elucidated, for the first time, that the glaucothoe, metamorphose stage, does not feed. (1) The mouthparts of zoea, glaucothoe and juveniles were compared. The mandibles of the glaucothe, especially the lateral lobes showed reduction in size. The gnathal processes are uncalcified, lacking denticles. The endites and endopods of the maxillae are rudimentally having minute setae. (2) The setae which presented in the foregut of zoea are reduced and the filter press is poorly developed in glaucothoe. The complex gastric mill is absent, which is well developed in the first juvenile. These facts suggest that feeding and food processing are not functional in the glaucothoe.The newly hatched zoea has lipid reserve in the epithelial cells (resorptive cells) of the midgut gland. Accumulation of lipids is … More observed in the medial portion of the midgut gland with feeding cultured diatom Thalassiosira sp. and Artemia nauplii during all zoea stages. The large lipid droplets obseved in the early glaucothoe disappeared in the late glaucothoe. Small lipid droplets distribute abundantly in the outer margin of the epithelial cells of the midgut gland tables in the late glaucothoe. Fatty acids analysis showed an increase of neutral and polar lipids contents at 41 and 84 ug/dry weight mg for the newly hatched zoea of P.camtschaticus to about 100 and 110 ug/mg at the final zoea and the early glaucothoe. The lipids content decreased to 60 and 80 ug/mg at the mid-stage glaucothoe, 25, 50 ug/mg at the late-stage, and 15.30 ug/mg at the first juvenile Neutral lipids content increased/decreased remarkably compared to polar lipids content. These facts indicate that the lipids are used as energy source during the non-feeding glaucothoe stage. The fat body, which presents in the hemocoel of the puerulus, was not observed in the glaucothoe. This may imply relatively short distance migration in glaucothoe compared to long distance migration in puerulus during coastal recruitment. Less

Paralithodes camtschaticus 和 P.brevipes（十足目：Anomura：石蟹科：Paralithodes）是北太平洋和鄂霍次克海中具有重要商业价值的帝王蟹。本研究首次阐明，变态阶段的白藻不进食。 (1)幼虫、白斑幼虫的口器比较。青光眼的下颌骨，特别是侧叶的尺寸缩小。颚突未钙化，缺乏小齿。上颌骨的内突和内足基本具有微小的刚毛。 (2)白斑幼虫前肠刚毛减少，压滤机发育不良。复杂的胃磨不存在，这在第一个青少年中发育良好。这些事实表明，蓝藻的摄食和食物加工功能不起作用。新孵化的幼虫在中肠腺的上皮细胞（吸收细胞）中具有脂质储备。在喂食培养的硅藻 Thalassiosira sp 的情况下，在中肠腺的内侧部分观察到脂质的积累。和卤虫无节幼体在所有幼虫阶段。早期白斑中观察到的大脂滴在晚期白斑中消失。小脂滴大量分布在晚期白斑中肠腺台上皮细胞的外缘。脂肪酸分析表明，新孵化的P.camtschaticus幼虫的中性和极性脂质含量增加到41和84ug/干重mg，而最终幼虫和早期白幼虫的中性和极性脂质含量增加到约100和110ug/mg。白鳢中期的脂质含量分别降至60和80 ug/mg，后期为25、50 ug/mg，幼年期为15.30 ug/mg。与极性脂质含量相比，中性脂质含量显着增加/减少。这些事实表明，脂质在非摄食白蝽阶段被用作能量来源。存在于幼稚血腔中的脂肪体在白癜风中未观察到。这可能意味着，与沿海招募过程中幼树的长距离迁移相比，白花的迁移距离相对较短。较少的

项目成果

F.A.Abrunhosa and J.Kittaka: "Morphological changes in the midgut, midgut gland and hindgut during the larval and postlarval develop-ment of the red king crab Paralithodes camtschaticus." Fisheries Science. 63(5). 746-754 (1997)

F.A.Abrunhosa 和 J.Kittaka：“红帝王蟹 Paralithodes camtschaticus 幼虫和幼虫后发育过程中中肠、中肠腺和后肠的形态变化。”

B.G.Stevens and J.Kittaka: "Postlarval settling behaviour, substrate preference and time to metamorphosis for red king crab Paralithodes camtschaticus." Marine Ecology Progress Series. 167. 197-206 (1998)

B.G.Stevens 和 J.Kittaka：“红王蟹 Paralithodes camtschaticus 的幼体沉降行为、基质偏好和变态时间。”

M.Ishikawa, S-i.Teshima, A.Kanazawa and S.Koshio: "Measurements of digestibilities of cholesterol and fatty acids using 5a-cholestane as an inert marker in the tilapia, Oreochromis niloticus, and the freshwater prawn, Macrobrachium rosenbergii." J.Appl.Ic

M.Ishikawa、S-i.Teshima、A.Kanazawa 和 S.Koshio：“使用 5a-胆甾烷作为罗非鱼、Oreochromis niloticus 和淡水虾、罗氏沼虾的惰性标记物测量胆固醇和脂肪酸的消化率。”

J.Kittaka,K.Ono,and J.Booth: "Complete development of green rock lobster Jasus verreauxi from egg to juvenile stage." Bulletin of Marine Science. 61(1). 57-71 (1997)

J.Kittaka、K.Ono 和 J.Booth：“绿岩龙虾 Jasus verreauxi 从卵到幼年阶段的完整发育。”

J.Kittaka: "Application of ecosystem culture medhod for complete development of phyllosomas of spiny lobster" Aquaculture. 155. 319-331 (1997)

J.Kittaka：“应用生态系统养殖方法实现大龙虾叶状体的完全发育”水产养殖。