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Role of unsaturated fatty acids in brown adipose tissue function - with special reference to docosahexaenoic acid.

不饱和脂肪酸在棕色脂肪组织功能中的作用 - 特别是二十二碳六烯酸。

基本信息

批准号：
09470014
负责人：
KUROSHIMA Akihiro
金额：
$ 6.78万
依托单位：
ASAHIKAWA MEDICAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

In an attempt to elucidate a role of membrane phospholipid n-3 polyunsaturated fatty acid (DHA) , the changes in DHA of rat brown adipose tissue (BAT) were studied under various conditions. (1) DHA and arachidonic acid (AA) were higher during neonatal period with higher BAT thermogenesis than in the adults. (2) DHA-supplemented diet increased BAT-DHA but decreased AA, resulting in the unchanged or decreased BAT in vitro thermogenesis. (3) DHA-deficient diet decreased BAT-DHA but not AA without BAT in vitro thermogenesis. (4) DHA plus AA-supplemented diet increased both fatty acids in BAT but did not change BAT in vitro thermogenesis. However, uncoupling protein 1 mRNA expression increased. (5) Chronic administration of nitric oxide synthase L-NAME did not change BAT-DHA, but decreased BAT in vitro thermogenesis due to decreased cellularity. (6) Heat acclimation or fasting decreased BAT-DHA and in vitro thermogenesis with correlation between these both parameters. (7) Methimazole-induced hypothyroidism decrease both DHA and AA, causing the decrease in BAT in vitro thermogenesis. Triiodothyronine-induced hyperthyroidism increased BAT in vitro thermogenesis due to hyperplasia without the changes of DHA. (8) Low rearing temperature during the neonatal to young period effected the long-lasting improved BAT in vitro thermogenesis as well as systemic nonshivering thermogenesis in later life. These findings suggest that DHA subserves as one of controlling factors of BAT thermogenic function.

为了阐明膜磷脂n-3多不饱和脂肪酸（DHA）的作用，研究了不同条件下大鼠棕色脂肪组织（BAT）中DHA的变化。(1)DHA和花生四烯酸（AA）在新生期较高，BAT产热高于成人。(2)补充DHA的饲料增加了BAT-DHA，但减少了AA，导致BAT在体外产热作用不变或减少。(3)DHA缺乏的饮食减少BAT-DHA，但没有BAT的AA在体外产热。(4)添加DHA和AA的饮食增加了BAT中的两种脂肪酸，但没有改变BAT体外产热。解偶联蛋白1 mRNA表达增加。(5)长期给予一氧化氮合酶L-NAME没有改变BAT-DHA，但由于细胞结构减少而降低了BAT体外产热。(6)热习服或禁食降低了BAT-DHA和体外产热，这两个参数之间存在相关性。(7)甲巯咪唑诱导的甲状腺功能减退症减少DHA和AA，导致BAT在体外产热减少。三碘甲状腺原氨酸诱导的甲状腺功能亢进症增加BAT在体外产热由于增生没有DHA的变化。(8)在新生儿到青年时期的低饲养温度影响持久的改善BAT体外产热以及在以后的生活中的全身非颤抖性产热。提示DHA是BAT产热功能的控制因素之一。