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Metabolic evaluation using in vivo NMR spectroscopy for individual organs under serious conditions associated with reductive liver mitochondrial state

在与还原肝线粒体状态相关的严重条件下使用体内核磁共振波谱对各个器官进行代谢评估

基本信息

批准号：
09307024
负责人：
OZAWA Kazue
金额：
$ 18.88万
依托单位：
SHIGA UNIVERSITY OF MEDICAL SCIENCE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09307024/
关键词：
AKBR liver mitochondria systemic care in vivo NMR spectroscopy energy metabolism アポトーシス ^<31>P NMR ^<13>C NMR

项目摘要

Even in severe circulatory insufficient conditions during hemorrhage in jaundiced rats, the phosphoenergetic level in the myocardium was maintained fairly well. In such conditions, arterial ketone body ratio (AKBR) decreased and it was found to be a potential parameter to evaluate the systemic metabolic state. We also applied ィイD131ィエD1P- and ィイD113ィエD1C-NMR spectroscopy together to examine the metabolic state of the liver. Furthermore, the metabolic states of individual organs, such as liver, heart, brain and skeletal muscles, were investigated with NMR spectroscopy using hemorrhagic shock, endotoxic shock, myocardial infarction, brain infarction, epilepsy and diabetic animal models. We incorporated ultra-fast echo planar imaging (EPI) technique into ィイD11ィエD1H-NMR spectroscopic imaging. We have already reported fast ィイD113ィエD1C metabolite mapping derived from ィイD113ィエD1C-glucose in rat focal brain ischemia.These NMR spectroscopic techniques were also applied to the clinical cases. In patients with diabetic gangrene, we found metabolic abnormalities in the skeletal muscles associated with increased pII. Such findings were clearly different from that with ischemia. For heart disease, ィイD131ィエD1P NMR spectroscopy during hand-grip exercise was found to be useful to judge the viability of myocardium. In brain research ィイD11ィエD1H NMR spectroscopy was carried out for the judgment of malignant potential of maningioma, the pathophysiologic condition of myotonic dystrophy, spectroscopy during photic stimulation was used for the prediction of the effects of lithium or electrical stimulation therapy for bipolar disorder. Fast NMR spectroscopic imaging technique could be also applicable with clinical cases.

即使在黄疸大鼠出血期间严重循环不足的情况下，心肌中的磷酸能水平也维持得相当好。在这种情况下，动脉酮体比率（AKBR）下降，它被发现是评估全身代谢状态的潜在参数。我们还应用ィイD131ィエD1P-和ィイD113ィエD1C-NMR波谱一起检查肝脏的代谢状态。此外，还利用失血性休克、内毒素休克、心肌梗塞、脑梗塞、癫痫和糖尿病动物模型，通过核磁共振波谱研究了肝脏、心脏、大脑和骨骼肌等各个器官的代谢状态。我们将超快回波平面成像（EPI）技术融入到ィイD11ィエD1H-NMR 光谱成像中。我们已经报道了大鼠局灶性脑缺血中源自ィイD113ィエD1C-葡萄糖的快速ィイD113ィエD1C代谢图谱。这些NMR波谱技术也应用于临床病例。在糖尿病坏疽患者中，我们发现骨骼肌代谢异常与 pII 增加相关。这些发现与缺血的发现明显不同。对于心脏病来说，握力运动期间的ィイD131ィエD1P NMR波谱被发现有助于判断心肌的活力。在脑研究中，ィイD11ィエD1H NMR波谱用于判断脑膜瘤的恶性潜能、强直性肌营养不良的病理生理状况，光刺激期间的波谱用于预测锂或电刺激治疗双相情感障碍的效果。快速核磁共振波谱成像技术也适用于临床病例。