MOLECULAR ENVIRONMENT OF THE ACTION SITE OF ANESTHETICS

麻醉剂作用部位的分子环境

• 批准号: 09307036
• 负责人: OGLI Kenji
• 金额: $ 8.96万
• 依托单位: KAGAWA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09307036/
• 关键词: ANESTHETICS; ANESTHETIC MECHANISMS; NON-SPECIFIC BINDING; MOLECULAR ENVIRONMENT; 分子環境; ファンデルワールスカ; 構造水; 両親媒性領域; 麻酔薬作用機序; site specific; non-site specific; 膜の極性; 膜濃縮過程; 両親媒性ドメイン; 膜蛋白質

More than a hundred years have passed since the study of mechanisms of anesthesia began. Overton's lipid theory has been the most fundamental theory for physicochemists in this field. However, this theory only dealt with how the anesthetic molecules exist in the action site, and did not explain the mechanisms of their action. Why are the concentrations of anesthetics that affect the physicochemical parameters of lipid membranes much higher than the clinical concentrations? On the other hand some effects of anesthetics on proteins can be observed at clinical concentrations. Therefore the use of proteins such as neurotransmitters and/or receptors for the study of anesthetic mechanisms is becoming popular. There have been many papers published regarding the "specific" action of anesthetics on proteins.In order to obtain a valuable theory, it is necessary to analyze a wide range of research. Therefore, it is important to continue physicochemical studies as well as biochemical studies. This project includes both physicochemical and biochemical investigators. During the project, we met together several times and discussed about how the environment around anesthetic at the action site is. To extend the discussion, we held an international workshop. Inviting many researchers from various fields concerning anesthetic mechanism, we have had a very meaningful talk.

麻醉机制的研究已经有一百多年了。奥弗顿脂质理论一直是该领域物理化学家最基本的理论。然而，这一理论只涉及麻醉分子如何存在于作用部位，并没有解释其作用机制。为什么影响脂质膜理化参数的麻醉药浓度远高于临床浓度？另一方面，在临床浓度下可以观察到麻醉剂对蛋白质的一些影响。因此，利用蛋白质如神经递质和/或受体来研究麻醉机制正变得越来越流行。关于麻醉药对蛋白质的“特异性”作用，已经发表了许多论文。为了获得有价值的理论，有必要对广泛的研究进行分析。因此，在进行生物化学研究的同时，也要继续进行理化研究。这个项目包括物理化学和生物化学研究人员。在项目过程中，我们多次见面，讨论了麻醉作用部位周围的环境。为了扩大讨论范围，我们举行了一次国际研讨会。我们邀请了来自麻醉机制各个领域的研究人员，进行了非常有意义的讨论。

项目成果

S. Sawamura: "Effect of Pressure on the Solubilities of Anthracene and Phenanthrene in Water at 298.15 K"J. Solution Chem.. 29. 369-376 (2000)

S. Sawamura：“压力对 298.15 K 下蒽和菲在水中溶解度的影响”J。

Ed.K.Ogli: "Progress of Anesthetic Mechanisms Vol.6 Special issure"Research Group of Anesthetic Mechanism in Japan. 660 (2000)

Ed.K.Ogli：《麻醉机制进展第6卷特刊》日本麻醉机制研究组。

K. Tamura and S. Kishioka: "Effects of low-power He-Ne laser on the growth of yeast under high pressure"Res. High Press. Sci. Tech.. Vol. 7. 1324-1326 (1998)

K. Tamura 和 S. Kishioka：“低功率氦氖激光对高压下酵母生长的影响”Res。

H.Satake: "Membrane-Buffer Partition Coefficients of a local Anesthetic Tetracaine Monitored by an Anesthetic Sensor ; Effect of Temperature and pH" Toxicol.Lett.100-101. 441-445 (1998)

H.Satake：“麻醉传感器监测局部麻醉丁卡因的膜缓冲液分配系数；温度和 pH 值的影响”Toxicol.Lett.100-101。

S.Sawamura: "Anomalous Pressure Dependence of the Solubility of C60 in n-Hexane" Chem.Phys.Lett.299. 177-179 (1999)

S.Sawamura：“C60 在正己烷中溶解度的异常压力依赖性”Chem.Phys.Lett.299。