Anesthetic effects on the physicochemical parameters and the functions of phase boundary of lipid membrane and macro-molecule

麻醉对脂质膜与大分子理化参数及相界功能的影响

基本信息

批准号：
03304039
负责人：
OGLI Kenji
金额：
$ 3.52万
依托单位：
KAGAWA MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03304039/
关键词：
Anesthetics Mechanism Membrane Macro-Molecules Interface

项目摘要

Anesthetics are not located in membrane core,but in interfacial region. On increasing the concentration, anesthetic molecules penetrate into the inside of membrane. By the application of Taft's polarity parameters to the spectral changes of probes incorporated in lipid membrane,it is revealed that anesthetics increase the H-bond acceptability and decrease the polarity at the interface. This effect can be explained by the release of hydrated water molecules from the membrane interface. Physicochemical properties concerning adsorption were also analyzed using charcoal. Alcohol and pressure synergistically induce the interdigitation in the lipid bilayer. This action of alcohol is not pressure reversible.Partitioninng into the membrane and cutoff phenomena: The thermal phase transition of lipid membrane consists of pre-transition from gel II to gel I and main-transition from gel I to liquid phase. Anesthetics are known to depress the phase transition temperatures. This effect is more significant with respect to the pre-transition. Under the condition that anesthetic potency is proportional to its oil solubility,the partition coefficients of anesthetics to liquid phase;K(L) are larger than those to gel I phase;K(G). When the number of carbon atoms of anesthetics increases, K(G) becomes larger than K(L). This may be the reason to cause cutoff phenomena of anesthesia.Effects on protein: Various informations about the effect of anesthetics upon the alpha-herix-beta-sheet transition of poly-L-lysine,and the activation of alpha-chymotrypsin were revealed.Conclusion: Many reports dealt with the site specific action of anesthetics were published in these days. However,from our considerations, most of these data can be treated as the results of non-specific actions of anesthetics to the macro-molecules. More communications between researchers and more cooperative research projects are desired.

麻醉药不是位于膜核，而是位于界面区域。在增加浓度时，麻醉分子渗透到膜的内部。通过将TAFT的极性参数应用于脂质膜中掺入的探针的光谱变化，揭示了麻醉药会增加H键的可接受性并降低界面处的极性。可以通过从膜界面释放水合水分子来解释这种效果。还使用木炭分析了有关吸附的物理化学特性。酒精和压力协同诱导脂质双层中的互钉。酒精的这种作用不是压力可逆的。进入膜和截止现象：脂质膜的热相转变包括从凝胶II到凝胶I到凝胶I以及从凝胶I到液相的主要过渡。已知麻醉剂会降低相变温度。相对于前过渡，这种效果更为重要。在麻醉效力与其油溶解度成正比的条件下，麻醉剂与液相的分配系数； K（L）大于凝胶I期； K（g）。当麻醉剂的碳原子数量增加时，k（g）会大于K（l）。这可能是引起麻醉现象的原因。对蛋白质的影响：关于麻醉药对多l-赖氨酸的α-herix-beta-sheet过渡的影响的各种信息，揭示了α-偶联蛋白酶的激活。结论：许多报道都与这些日期相关的是与这些日期相关的，这些报道是在这些日期的特定于这些日期的杂物。但是，从我们的考虑因素来看，这些数据中的大多数可以被视为麻醉药对宏观分子的非特异性作用的结果。需要更多的研究人员与更多合作研究项目之间的沟通。