Drug and Species Differences of the Effects of Inhalational Anesthetics on the Behavior and / or Biological Response of Animals

吸入麻醉药对动物行为和/或生物反应影响的药物和物种差异

基本信息

批准号：
63480349
负责人：
OGLI Kenji
金额：
$ 4.16万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Species differences were recognized on the effects of volatile anesthetics in Drosophila melanogasters and mice.Information from Drosophila study :The Gene which controls senditivity to an agent might be different from those to other agents, from the studies on ether, choloroform and halothane. An ether tolerant mutant was radiosensitive but it is not universal. Generally, ether anesthesia nonspecifically decreases the effect of radiation. Furthermore, the mutant of Na^+ channel, para ts2, is hypersensitive to ether anesthesia and it is suspected from the binding of para locus that the hypersensitivity links to any change of channel protein structure.Information from mouse study :Anesthetic induced opisthotonus was highly frequently found in ddN and YBR, and seldom found in C57BL and R3/IMR/Ki among 13 strains of mouse. The height of incidence due to anesthetics is in order of sevoflurane, isoflurane, enflurane, methoxyflurane and halothane. And the high incidence is also proportionate to the low value of blood/gas partition coefficient. In avoidance training, memory consolidation and retention are promoted by inhalation anesthesia. Central nervous system excitation of opisthotonus and memory promoting are not attributable to the same mechanism. Furthermore, strain-difference of sensitivity to volatile anesthetics was found. ddN strain showed higher ED_<50> of loss of righting reflex, C57BL showed lower ED_<50>. And F1 hybrid mice by reciprocal crossing between the two strains showed intermediate value in sevoflurane, enflurane and isoflurane. In halothane. individual ED_<50> was in the same range of value. The values of ED_<50> of F2 hybrid in Isoflurane and enflurane were in wide range. Free amino acids and catecholamines of mouse brain showed significant differences among strains, but these differences were not directly related to the sensitivity of anesthesia.

物种差异对果蝇和小鼠的挥发性麻醉剂的影响得到了认识到。果蝇研究的信息：控制对药剂的发送效率的基因可能与其他药物的差异不同，与其他对以太，胆固醇和晕阳光的研究相比。乙醚耐受性突变体是放射敏感的，但不是普遍的。通常，乙醚麻醉非特异性降低了辐射的影响。此外，Na^+通道的突变体Para TS2对乙醚麻醉是高度敏感的，并且从Para基因座的结合中怀疑，超敏反应与通道蛋白结构的任何变化：麻醉诱导的opisthotonus在DDN和SELD NOM中很常见，并且在C5/ybr中很常见。鼠标。麻醉剂引起的发病率是按照七氟，异氟烷，enflurane，甲氧基氟烷和硫烷的顺序。并且高发病率也与血/气体分配系数的低价值成正比。在回避训练中，记忆巩固和保留率是通过吸入麻醉来促进的。中枢神经系统的兴奋和促进记忆并不归因于相同的机制。此外，还发现了对挥发性麻醉剂的敏感性的应变差异。 DDN菌株显示出更高的ED_ <50>骨质反射的损失，C57BL显示出较低的ED_ <50>。通过两种菌株之间的相互交叉交叉的F1杂种小鼠在Sevoflurane，Enflurane和Isoflurane中显示出中间值。在Halothane。单个ed_ <50>在相同的值范围内。异氟烷和Enflurane中F2杂交的ED_ <50>的值在较大范围内。小鼠脑的游离氨基酸和儿茶酚胺在菌株之间显示出显着差异，但是这些差异与麻醉的敏感性并不直接相关。