Molecular Mechanisms Underlying Sperm-Eng Interaction

精子与工程相互作用的分子机制

• 批准号: 09044210
• 负责人: HOSHI Motonori
• 金额: $ 3.39万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044210/
• 关键词: fertilization; acrosome reaction; sperm; oocyte; cGMP; 棘皮動物; 精子活性化ペプテド; 卵・精子結合; 糖鎖; キヒトデ

In starfish Asterias amurensis, three components in the jelly coat of eggs, namely acrosome reaction-inducing substance (ARIS), Co-ARIS and asterosap, act in concerted on homologous spermatozoa to elicit the acrosome reaction species-specifically. ARIS is proteoglycan-like compound which binds to the receptor localized to a small domain in the anterior portion of sperm heads. Its activity is mostly attributable to a sulfatedpolysaccharide chain composed of one thousand or so repeating units of a sulfated pentasaccharide. Co-ARIS is a group of saponins consisting of a sulfated steriod and a pentasaccharide. In combination with ARIS, it increases intracellular CaィイD12+ィエD1 concentration of sperm. Asterosap is a group of glutamine-rich tetratricontapeptides with a disulfide linkage. It increases cGMP level and intracellular pH of sperm through binding to its receptor that is the guanylate cyclase of 125 kDa in a major sperm membrane protein.

海星（Asterias amurensis）卵胶状被膜中的顶体反应诱导物质（acrosome reaction-inducing substance， ARIS）、Co-ARIS和asterosap三种成分协同作用于同源精子引起顶体特异性反应。ARIS是一种类似蛋白聚糖的化合物，它与位于精子头部前部的一个小区域的受体结合。它的活性主要是由于一个硫酸化的多糖链，由一个硫酸化的五糖的1000个左右的重复单位组成。Co-ARIS是一组由硫酸化的甾体和五糖组成的皂苷。与ARIS联合使用时，可增加精子胞内Ca (γ) - D12+ γ (γ) - D1浓度。Asterosap是一组富含谷氨酰胺的四联肽，具有二硫键。它通过与精子主要膜蛋白中125 kDa的鸟苷酸环化酶受体结合，增加精子的cGMP水平和胞内pH值。

项目成果

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Midori Matsumoto: "Sequence Analysis of cDNAs Encoding Precursors of Starfish asterosaps"Developmental Genetics. 25. 130-136 (1999)

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Kazuyoshi Chiba: "Induction of Germinal vesicle breakdown in a cell-free preparation from stafish oocytes"Developmental Biology. 205. 217-223 (1999)

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