Structure and function of occludin in tight junctions : comparison with connexin gap junctions
紧密连接中occludin的结构和功能:与连接蛋白间隙连接的比较
基本信息
- 批准号:09044290
- 负责人:
- 金额:$ 4.1万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for international Scientific Research
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Occiudin-deficient embryonic stem (ES) cells were generated by targeted disruption of both alleles of the occiudin gene. When these cells were subjected to suspension culture, they aggregated to form simple, and then cystic embryoid bodies (EBs) with the same time course as EB formation from wild-type ES cells. Immunofluorescence microscopy and ultrathin section electron microscopy revealed that polarized epithelial (visceral endoderm-like) cells were differentiated to delineate EBs not only from wild-type but also from oceludin-deficient ES cells. Freeze fracture analyses indicated no significant differences in number or morphology of TI strands between wild-type and occludin-deficient epithelial cells. These findings indicate that there are as yet unidentified TJ integral membrane protein(s) which can form strand structures. We therefore re-examined the isolated junction fraction from chicken liver, from which occiudin was first identified. Among numerous components of this fraction, … More only a broad silver-stained band around 22 kD was detected with the occludin band through 4 M guanidine-HCI extraction as well as sonication followed by stepwise sucrose density gradient centrifugation. Two distinct peptide sequences were obtained from the lower and upper halves of the broad band, and similarity searches of data bases allowed us to isolate two full-length cDNAs encoding related mouse 22-kD proteins consisting of 211 and 230 a.a., respectively. Hydrophilicity analysis suggested that both bore four transmembrane domains, although they did not show any sequence similarity to occludin. Immunofluorescence and immunoelectron microscopy revealed that both proteins tagged with FLAG or GFP were targeted to and incorporated into the TJ strand itself. We designated them as "claudin-1" and "claudin-2", respectively. Although the precise structure/function relationship of the claudins to TI still remains elusive, these findings indicated that multiple integral membrane proteins with four putative transmembrane domains, occludin and claudins, constitute TJ strands. Less
Occiudin缺陷胚胎干细胞是通过定向破坏occiudin基因的两个等位基因而产生的。在悬浮培养下,这些细胞聚集成简单的囊状胚体(EBS),其形成过程与野生型ES细胞形成EB的过程相同。免疫荧光显微镜和超薄切片电子显微镜显示分化的极化上皮细胞(内脏内胚层样细胞)不仅可以区分野生型的EBS,还可以区分缺失oceludin的ES细胞。冷冻断裂分析表明,野生型和闭合素缺陷型上皮细胞在TI链的数量和形态上没有显著差异。这些发现表明,目前尚未鉴定的TJ完整膜蛋白(S)可以形成链结构。因此,我们重新检测了从鸡肝中分离出的连接组分,从其中首次鉴定出了occiudin。在该组分的众多成分中,…此外,通过4M盐酸胍提取、超声和蔗糖密度梯度离心法,只在22kD左右检测到一条银染的条带,并带有闭锁蛋白条带。从宽带的下半部分和上半部分获得了两个不同的肽序列,通过数据库的相似性搜索,我们分离到了两个编码相关的小鼠22-kD蛋白的全长cDNA,分别由211和230个氨基酸组成。亲水性分析表明,它们都有四个跨膜结构域,尽管它们没有显示出任何与occludin相似的序列。免疫荧光和免疫电子显微镜显示,两种标记有FLAG或GFP的蛋白质都以TJ链本身为靶标并被掺入。我们将它们分别命名为“claudin-1”和“claudin-2”。虽然Claudins与TI的确切结构/功能关系仍然不清楚,但这些发现表明,TJ链由具有四个假定的跨膜结构域的多个整膜蛋白组成,即occludin和claudins。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sachiko TSUKITA: "ERM proteins : Head-to-tail regulation of actin/plasma membrane regulations." Trends in Biological Science. 22. 53-58 (1997)
Sachiko TSUKITA:“ERM 蛋白:肌动蛋白/质膜调节的头尾调节。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Saitou,M.,Fujimoto,K.,Doi,Y.,Itoh,M.,Fujimoto,T.,Furuse,M.,Takano,H.,Noda,T.and Tsukita,Sh.: "Occludin-deficient embryonic stem cells can diferentiate into polarized epithelial cells bearing tight junctions" J.Cell Biol.141. 297-408 (1998)
Saitou,M.,Fujimoto,K.,Doi,Y.,Itoh,M.,Fujimoto,T.,Furuse,M.,Takano,H.,Noda,T.和 Tsukita,Sh.:“Occludin 缺陷胚胎
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakakibara,A.,Furuse,A.,Saitou,M.,Ando-Akatsuka,Y.,and Tsukita,Sh.: "Possible involvement of phosphorylation of occludin in tight junction formation" J.Cell Biol.137. 1393-1401 (1997)
Sakakibara,A.、Furuse,A.、Saitou,M.、Ando-Akatsuka,Y. 和 Tsukita,Sh.:“occludin 磷酸化可能参与紧密连接形成”J.Cell Biol.137。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Takahisa KONDO: "ERM (Ezrin/Radixin/Moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis." Journal of Cell Biology. 139. 758-767 (1997)
Takahisa KONDO:“细胞凋亡早期阶段微绒毛破坏的基于 ERM(Ezrin/Radixin/Moesin)的分子机制。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Furuse,M.,Sasaki,H.,Fujimoto,K.,and Tsukita,Sh: "A single gene product,claudin-1 or -2,reconstitutes tight junction strands and recruits occludin in fibroblasts." J.Cell Biol.143. 391-401 (1998)
Furuse,M.、Sasaki,H.、Fujimoto,K. 和 Tsukita,Sh:“单基因产物claudin-1 或-2,可重建紧密连接链并在成纤维细胞中招募occludin。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TSUKITA Shoichiro其他文献
TSUKITA Shoichiro的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TSUKITA Shoichiro', 18)}}的其他基金
Claudins in the epithelium/endothelium barrier dysfucrition
上皮/内皮屏障功能障碍中的 Claudins
- 批准号:
14207008 - 财政年份:2002
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular mechanism for cell-cell adhesion in canceration and metastasis
癌变和转移中细胞粘附的分子机制
- 批准号:
12219210 - 财政年份:2000
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
The claudin family : Its involvement in interecellular sealing and epithelial polarity
密蛋白家族:参与细胞间密封和上皮极性
- 批准号:
11307002 - 财政年份:1999
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
CLAUDINS AND OCCLUDIN : COMPARISON WITH CONNEXIN
CLAUDINS 和 OCCLUDIN:与 CONNEXIN 的比较
- 批准号:
11694270 - 财政年份:1999
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Development of a new drug delivery method by the use of occludin molecules
利用occludin分子开发新的药物递送方法
- 批准号:
10557011 - 财政年份:1998
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The structure and functions of occludin
occludin的结构和功能
- 批准号:
08407006 - 财政年份:1996
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
MODULATION OF BLOOD-BRAIN BARRIER
血脑屏障的调节
- 批准号:
06557014 - 财政年份:1994
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
CELL ADHESION-DEPENDENT REGULATION OF CELL GROWTH AND DIFFERENTIATION
细胞生长和分化的细胞粘附依赖性调节
- 批准号:
06404083 - 财政年份:1994
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Time-lapse Electron Microscopy with Caged Compounds
笼状化合物的延时电子显微镜
- 批准号:
04558034 - 财政年份:1992
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
A New Rapid Freezing Apparatus for Electron Microscopy
一种新型电子显微镜快速冷冻装置
- 批准号:
02558026 - 财政年份:1990
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
相似国自然基金
基于GAPR-1/Occludin信号通路探讨培土
清心方调控自噬恢复皮肤屏障治疗特应
性皮炎的作用机制研究
- 批准号:
- 批准年份:2025
- 资助金额:10.0 万元
- 项目类别:省市级项目
Occludin调控内皮细胞紧密连接损伤在动脉粥样硬化中的作用及机制研究
- 批准号:82370447
- 批准年份:2023
- 资助金额:49 万元
- 项目类别:面上项目
通过Clathrin/AP2途径介导内皮细胞Occludin自噬性降解以靶向开放胶质瘤血肿瘤屏障的机制研究
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
罗伊氏乳杆菌通过MAPK通路上调断奶仔猪空肠上皮细胞Occludin基因转录的机制研究
- 批准号:n/a
- 批准年份:2022
- 资助金额:0.0 万元
- 项目类别:省市级项目
血清31-kDa Occludin降解片段作为预测缺血性脑卒中血管再通治疗脑出血风险的新型标志物及其降解机制研究
- 批准号:n/a
- 批准年份:2022
- 资助金额:10.0 万元
- 项目类别:省市级项目
从PAR1/2-MMP2/3通路探求自诱导分子2调控紧密连接蛋白occludin对新生儿坏死性小肠结肠炎的影响及机制研究
- 批准号:
- 批准年份:2021
- 资助金额:0.0 万元
- 项目类别:省市级项目
紧密连接蛋白Occludin参与牛病毒性腹泻病毒复制的分子机制研究
- 批准号:32160829
- 批准年份:2021
- 资助金额:35 万元
- 项目类别:地区科学基金项目
紧密连接蛋白Occludin在抗RNA病毒天然免疫应答中作用和机制研究
- 批准号:
- 批准年份:2020
- 资助金额:58 万元
- 项目类别:面上项目
支气管哮喘发病中MUC1缺失通过NEDD4-2促进Occludin泛素化导致气道上皮屏障功能紊乱的机制研究
- 批准号:82060007
- 批准年份:2020
- 资助金额:34 万元
- 项目类别:地区科学基金项目
SNHG12结合Occludin抑制其泛素化维系脑缺血应激损伤中血脑屏障结构稳态的研究
- 批准号:31900826
- 批准年份:2019
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Alcohol increases tight junction permeability & disrupts translocation of ZO-1
酒精会增加紧密连接的通透性
- 批准号:
8306369 - 财政年份:2011
- 资助金额:
$ 4.1万 - 项目类别:
Alcohol increases tight junction permeability & disrupts translocation of ZO-1
酒精会增加紧密连接的通透性
- 批准号:
8127121 - 财政年份:2011
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
8323545 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
8528567 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
8101858 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
8587380 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
8733675 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Tight Junction: Gate to HCV Tropism, Therapeutics and Pathogenesis
紧密连接:HCV 趋向性、治疗和发病机制的大门
- 批准号:
7943456 - 财政年份:2010
- 资助金额:
$ 4.1万 - 项目类别:
Regulation of tight-junction in gastrointestinal epithelia by clausin-WNK system
克劳辛-WNK系统对胃肠道上皮细胞紧密连接的调节
- 批准号:
18790454 - 财政年份:2007
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Expression patterns of tight junction associated proteins, occludin and claudins in ameloblasts during amelogenesis of mouse incisor.
小鼠门牙成釉细胞中紧密连接相关蛋白、occludin 和claudins 的表达模式。
- 批准号:
17591909 - 财政年份:2005
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (C)














{{item.name}}会员




